Marcol Wiesław, Kotulska Katarzyna, Larysz-Brysz Magdalena, Kowalik Joanna Lewin
Department of Physiology, Medical University of Silesia, 18 Medyków St., 40-752, Katowice, Poland.
Neurosurg Rev. 2007 Jul;30(3):235-43; discussion 243. doi: 10.1007/s10143-007-0085-5. Epub 2007 May 26.
The outcome of peripheral nerve injury is often impaired by neuropathic pain, which is resistant to most analgesics and presents a serious clinical problem. The mechanisms underlying post-traumatic neuropathic pain remain unclear, but they are likely associated with the regeneration processes. Brain-derived neurotrophic factor (BDNF) is known to enhance peripheral nerve regeneration and is also considered to be an endogenous modulator of nociceptive responses following spinal cord lesion. The aim of this work was to examine the local effect of BDNF in a neuropathic pain model. Sciatic nerves of adult male rats were transected and supplied with connective tissue chambers filled with (1) fibrin only, (2) fibrin with BDNF, or (3) fibrin with antibodies against BDNF. In control animals the nerve was transected and no chamber was applied. During follow-up, autotomy behavior was assessed. Seven weeks after the operation, the number of surviving and regenerating neurons in dorsal root ganglia was counted and the neuroma incidence was examined. We found that local inactivation of BDNF decreased the incidence as well as severity of autotomy and neuroma formation, but did not influence neuron regeneration into the chambers. These results indicate that BDNF plays a locally crucial role in neuropathic pain development after peripheral nerve injury.
周围神经损伤的结果常因神经性疼痛而受损，这种疼痛对大多数镇痛药都有抗性，是一个严重的临床问题。创伤后神经性疼痛的潜在机制尚不清楚，但可能与再生过程有关。已知脑源性神经营养因子（BDNF）可促进周围神经再生，也被认为是脊髓损伤后伤害性反应的内源性调节因子。这项工作的目的是研究BDNF在神经性疼痛模型中的局部作用。将成年雄性大鼠的坐骨神经切断，并在其周围放置结缔组织腔，腔内填充（1）仅纤维蛋白、（2）含BDNF的纤维蛋白或（3）含抗BDNF抗体的纤维蛋白。对照组动物的神经被切断但不放置腔室。在随访期间，评估自切行为。术后7周，计数背根神经节中存活和再生神经元的数量，并检查神经瘤的发生率。我们发现，BDNF的局部失活降低了自切和神经瘤形成的发生率及严重程度，但不影响神经元向腔室内的再生。这些结果表明，BDNF在周围神经损伤后的神经性疼痛发展中起局部关键作用。
