使用微流控梯形腔在二维和三维环境中生成稳定的浓度梯度。

Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamber.

作者信息

Saadi Wajeeh, Rhee Seog Woo, Lin Francis, Vahidi Behrad, Chung Bong Geun, Jeon Noo Li

机构信息

Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Science II, Irvine, CA 92697, USA.

出版信息

Biomed Microdevices. 2007 Oct;9(5):627-35. doi: 10.1007/s10544-007-9051-9.

DOI:10.1007/s10544-007-9051-9

PMID:17530414

Abstract

We have developed a simple microfluidic device for generating stable concentration gradients in 2D and 3D environments. The device, termed the Ladder Chamber, uses a two-compartment diffusion system to generate steady state gradients across flow-free channels that connect the source and sink channels. To demonstrate the utility of the Ladder Chamber for cell migration, neutrophil chemotaxis was successfully observed in soluble chemoattractant (IL-8) gradient. The Ladder Chamber's simple design and experimental implementation make it an attractive approach for investigating cell migration and other biological experiments in well-defined gradients in 2D surfaces as well as in 3D gels.

摘要

我们开发了一种简单的微流控装置，用于在二维和三维环境中产生稳定的浓度梯度。该装置称为阶梯室，它使用两室扩散系统在连接源通道和汇通道的无流通道上产生稳态梯度。为了证明阶梯室在细胞迁移方面的实用性，我们在可溶性趋化因子（IL-8）梯度中成功观察到了中性粒细胞趋化性。阶梯室简单的设计和实验操作使其成为一种有吸引力的方法，可用于在二维表面以及三维凝胶中明确的梯度下研究细胞迁移和其他生物学实验。