[来自意大利石松种子的贮藏蛋白]

[Storage proteins from seeds of Pinus pinea L].

作者信息

Nasri Nizar, Triki Saïda

机构信息

Laboratoire de biochimie des protéines et des lipides, Campus universitaire, Tunis 2092, Tunisie.

出版信息

C R Biol. 2007 May;330(5):402-9. doi: 10.1016/j.crvi.2007.03.011. Epub 2007 May 9.

DOI:10.1016/j.crvi.2007.03.011

PMID:17531790

Abstract

The Mediterranean stone pine Pinus pinea L. (gymnosperm, Pinaceae) is much appreciated for its seed production, widely used in food preparation in the Mediterranean Basin. Seeds contain 25% proteins on a dry-weight basis. Pinus pinea accumulate globulins as major storage proteins in seeds (75% of total storage proteins), composed of several subunits of 10 to 150 kDa, revealed by SDS-PAGE. The albumin fraction (15%) represents three subunits of 14, 24 and 46 kDa. Glutelins, the least soluble fraction, represents a small proportion (10%). Their constitutive units have frequent PM of 43 kDa. Prolamins also represent a very small percentage (1 to 2%).

摘要

地中海石松（Pinus pinea L.，裸子植物，松科）因其种子产量而备受青睐，在地中海盆地广泛用于食品制备。种子干重基础上含有25%的蛋白质。地中海石松在种子中积累球蛋白作为主要储存蛋白（占总储存蛋白的75%），由SDS-PAGE显示的几种10至150 kDa的亚基组成。清蛋白部分（15%）由14、24和46 kDa的三个亚基组成。谷蛋白是溶解性最差的部分，占比很小（10%）。它们的组成单元常见分子量为43 kDa。醇溶蛋白占比也非常小（1%至2%）。