Boudjeko Thaddée, Ngomoyogoli Judith Emery Kanemoto, Woguia Alice Louise, Yanou Nicolas Njintang
Laboratory of Phytoprotection and Valorisation of Plants Resources, Biotechnology Centre - Nkolbisson, P,O, BOX 3851, Messa, Yaounde, Cameroon.
BMC Complement Altern Med. 2013 Dec 11;13:352. doi: 10.1186/1472-6882-13-352.
High fat diet is known to induce oxidative stress and abnormal changes in lipid metabolism. Many traditional plants have been shown to possess antioxidant and lipid-lowering activities, improving on oxidative status and lipid profile. In this paper, we characterized and examined the antioxidative properties of the oilseed cake of A. floribunda and J. curcas. We also evaluated their effect on lipid profile in the plasma and liver of experimental rats placed on a high fat diet.
For a partial characterization, the qualitative and quantitative analyses of storage proteins, dietary fibre and polyphenol content were evaluated. Four extracts (aqueous, ethanolic, methanolic and 0.1 N HCl) were evaluated for their antioxidant properties and scavenging activities. The effect on lipid profile was evaluated after the administration of the crude extracts to albino rats placed on a high fat diet.
Our results showed that J. curcas contains 10 times more storage proteins than A. floribunda while A. floribunda contains twice as much total dietary fibre than J. curcas. An evaluation of the different families of storage proteins showed that J. curcas has glutelins as the major storage proteins in its seeds (61.65 mg/g d.m), followed by globulins (25.30 mg/g d.m) and albumins (18.30 mg/g d.m). The electrophoretic analyses revealed a diversity of bands at the level of the different families and for both species. The evaluation of the in vitro antioxidant activities showed that A. floribunda extracts had higher antioxidant properties. Although the composition of A. floribunda and J. curcas oilseed cake are different, they lowered serum triglycerides (TG), total cholesterol (TC) and blood glucose level.
These results show that the oilseed cake of A. floribunda and J. curcas possess antioxidant properties with an effect on blood glucose level and lipid profile.
已知高脂饮食会引发氧化应激以及脂质代谢异常变化。许多传统植物已被证明具有抗氧化和降血脂活性，可改善氧化状态和血脂情况。在本文中，我们对多花野牡丹和麻疯树的油籽饼的抗氧化特性进行了表征和研究。我们还评估了它们对高脂饮食实验大鼠血浆和肝脏中血脂情况的影响。
为进行部分表征，对储存蛋白、膳食纤维和多酚含量进行了定性和定量分析。对四种提取物（水提取物、乙醇提取物、甲醇提取物和0.1 N盐酸提取物）的抗氧化特性和清除活性进行了评估。将粗提取物给予高脂饮食的白化大鼠后，评估其对血脂情况的影响。
我们的结果表明，麻疯树的储存蛋白含量比多花野牡丹多10倍，而多花野牡丹的总膳食纤维含量是麻疯树的两倍。对不同储存蛋白家族的评估表明，麻疯树种子中的主要储存蛋白是谷蛋白（61.65毫克/克干重），其次是球蛋白（25.30毫克/克干重）和白蛋白（18.30毫克/克干重）。电泳分析揭示了不同家族水平以及两种植物的条带多样性。体外抗氧化活性评估表明，多花野牡丹提取物具有更高的抗氧化性能。尽管多花野牡丹和麻疯树油籽饼的成分不同，但它们都降低了血清甘油三酯（TG）、总胆固醇（TC）和血糖水平。
这些结果表明，多花野牡丹和麻疯树的油籽饼具有抗氧化特性，对血糖水平和血脂情况有影响。
