在小鼠乳腺细胞中表达的传染性胃肠炎冠状病毒刺突蛋白诱导的免疫反应。

Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells.

作者信息

Peng Shu-Ying, Lv Ning, Zhang Yong, Bi Cong-Ming, Cao Jun-Wei, Li Zhi-Yan, Zhang Shi-Qiang, He Xiao-Ning

机构信息

Institute of Biotechnology, Northwest Agriculture and Forestry University, NO. 3 TaiCheng Road, Yangling 712100, PR China.

出版信息

Virus Res. 2007 Sep;128(1-2):52-7. doi: 10.1016/j.virusres.2007.04.004. Epub 2007 May 25.

DOI:10.1016/j.virusres.2007.04.004

PMID:17532081

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7114266/

Abstract

The present study is undertaken to investigate the immune response that was induced by the recombinant spike (S) protein from swine-transmissible gastroenteritis virus (TGEV) expressed in mouse mammary cells. A mammary-specific expression vector pEBS containing the full-length cDNA of S gene was constructed and expressed in the mouse mammary cells (EMT6). The recombinant S protein from culture supernatant of transgenic EMT6 was harvested and immunized BALB/c mice. The results demonstrated recombinant S protein was expressed at high levels in mammary cells by Western blotting and enzyme-linked immunosorbent assay (ELISA) detection. The antibody titer in BALB/c mice following immunization with recombinant S protein was detectable after the first immunization. Maximum titers of antibody (8.86+/-0.19 ng/ml of serum) were attained after the second immunization. In conclusion, the recombinant S protein expressed in mammary cells was able to elicit substantial immunological response against TGEV. This lays the basis for using mammary gland bioreactor generating edible vaccine.

摘要

本研究旨在探究由在小鼠乳腺细胞中表达的猪传染性胃肠炎病毒（TGEV）重组刺突（S）蛋白诱导的免疫反应。构建了包含S基因全长cDNA的乳腺特异性表达载体pEBS，并在小鼠乳腺细胞（EMT6）中表达。收获转基因EMT6培养上清中的重组S蛋白，并免疫BALB/c小鼠。通过蛋白质免疫印迹法和酶联免疫吸附测定（ELISA）检测表明，重组S蛋白在乳腺细胞中高水平表达。用重组S蛋白免疫后，BALB/c小鼠在首次免疫后即可检测到抗体效价。第二次免疫后抗体效价达到最高（血清中为8.86±0.19 ng/ml）。总之，在乳腺细胞中表达的重组S蛋白能够引发针对TGEV的显著免疫反应。这为利用乳腺生物反应器生产可食用疫苗奠定了基础。