杆状病毒表达的传染性胃肠炎病毒S蛋白的免疫原性
Immunogenicity of the S protein of transmissible gastroenteritis virus expressed in baculovirus.
作者信息
Tuboly T, Nagy E, Dennis J R, Derbyshire J B
机构信息
Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
出版信息
Arch Virol. 1994;137(1-2):55-67. doi: 10.1007/BF01311173.
Seven fragments of the spike (S) gene cDNA of transmissible gastroenteritis virus (TGEV), as well as the full length cDNA, were cloned and expressed in baculovirus vectors. Piglets were immunized with cells infected with the recombinant viruses. Each of the recombinants induced TGEV-specific antibodies detected in a fixed cell enzyme immunoassay. The amino terminal half of the S protein, containing all four major antigenic sites (A, B, C and D), and encoded by a 2.2 kb fragment of the S gene, induced virus neutralizing (VN) antibody titers comparable with those induced by the complete S protein. Recombinant proteins lacking the A antigenic site, or with a deletion including the putative receptor binding sites and the D antigenic site, were not capable of inducing levels of VN antibodies similar to those induced by the whole S protein.
传染性胃肠炎病毒（TGEV）刺突（S）基因cDNA的七个片段以及全长cDNA被克隆并在杆状病毒载体中表达。用感染重组病毒的细胞对仔猪进行免疫。每种重组体均诱导出在固定细胞酶免疫测定中检测到的TGEV特异性抗体。S蛋白的氨基末端一半包含所有四个主要抗原位点（A、B、C和D），由S基因的2.2 kb片段编码，诱导出的病毒中和（VN）抗体滴度与完整S蛋白诱导的滴度相当。缺乏A抗原位点或缺失包括假定受体结合位点和D抗原位点的重组蛋白不能诱导出与整个S蛋白诱导的VN抗体水平相似的水平。
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