Vihola Henna, Marttila Anna-Kaisa, Pakkanen Jukka S, Andersson Mirja, Laukkanen Antti, Kaukonen Ann Marie, Tenhu Heikki, Hirvonen Jouni
Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, PB 56, FIN-00014 Helsinki, Finland.
Int J Pharm. 2007 Oct 1;343(1-2):238-46. doi: 10.1016/j.ijpharm.2007.04.020. Epub 2007 Apr 27.
Cell-polymer interactions of thermosensitive poly(N-isopropylacrylamide) (PNIPAM) or poly(N-vinylcaprolactam) (PVCL) coated particles with RAW264.7 macrophages and intestinal Caco-2 cells were evaluated. Nanosized particles were prepared by modifying the surface of fluorescent polystyrene (FPS) particles with the thermosensitive polymer gels or with poly(ethylene oxide) (PEO)-macromonomer grafts. The particles were characterized by IR-spectroscopy for functional groups, light scattering for size distribution and zeta-potential for surface charge. Effects of temperature and polymer coating/grafting on the cellular interactions were evaluated by cell association/uptake and visualized by confocal scanning microscope. PEO and PNIPAM inhibited the polymer-cell contact by steric repulsion, evidenced by weak attachment of the particles. PVCL-coated FPS was adsorbed on the cells more strongly, especially at 37 degrees C, because of more hydrophobic nature at higher temperatures. The results suggest feasibility of the PNIPAM and PVCL for biotechnological/pharmaceutical applications, as the cell-particle interactions may be modified by size, surface charge, hydrophobicity, steric repulsion and temperature.
评估了热敏性聚（N-异丙基丙烯酰胺）（PNIPAM）或聚（N-乙烯基己内酰胺）（PVCL）包被的颗粒与RAW264.7巨噬细胞和肠Caco-2细胞之间的细胞-聚合物相互作用。通过用热敏聚合物凝胶或聚（环氧乙烷）（PEO）-大分子单体接枝修饰荧光聚苯乙烯（FPS）颗粒的表面来制备纳米颗粒。通过红外光谱表征颗粒的官能团，通过光散射表征尺寸分布，通过zeta电位表征表面电荷。通过细胞结合/摄取评估温度和聚合物包被/接枝对细胞相互作用的影响，并通过共聚焦扫描显微镜进行可视化。PEO和PNIPAM通过空间排斥抑制聚合物与细胞的接触，这通过颗粒的弱附着得到证明。PVCL包被的FPS在细胞上的吸附更强，尤其是在37℃时，因为在较高温度下其疏水性更强。结果表明PNIPAM和PVCL在生物技术/制药应用方面具有可行性，因为细胞-颗粒相互作用可通过尺寸、表面电荷、疏水性、空间排斥和温度进行调节。
