人博卡病毒（HBoV）的完整编码序列及系统发育分析
Complete coding sequences and phylogenetic analysis of Human Bocavirus (HBoV).
作者信息
Chieochansin Thaweesak, Chutinimitkul Salin, Payungporn Sunchai, Hiranras Thitikul, Samransamruajkit Rujipat, Theamboolers Apiradee, Poovorawan Yong
机构信息
Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
出版信息
Virus Res. 2007 Oct;129(1):54-7. doi: 10.1016/j.virusres.2007.04.022. Epub 2007 May 29.
Human Bocavirus (HBoV) is a novel virus which can cause respiratory tract disease in infants or children. Recently, the prevalence of this virus has been studied worldwide. In this study, 18 of 252 (7.14%) nasopharyngeal suctions from infants or children between 1 month and 9 years of age with respiratory tract illness were HBoV-positive by PCR. Three positive samples were selected for sequencing the entire coding sequences using a new conserved set of primers. The results showed that the most conserved regions of HBoV are the NS1 and NP1 genes, whereas VP1 and VP2 showed frequent variations. However, the complete coding sequences showed that the variations did not depend on the origin of virus found. The complete coding sequences determined in this study can resolve the problem of an HBoV detection method, which can be advantageously implemented in laboratory detection.
人博卡病毒（HBoV）是一种可导致婴幼儿或儿童呼吸道疾病的新型病毒。最近，该病毒在全球范围内的流行情况已得到研究。在本研究中，252份来自1个月至9岁患有呼吸道疾病的婴幼儿或儿童的鼻咽抽吸物中，有18份（7.14%）经聚合酶链反应（PCR）检测为HBoV阳性。选择3份阳性样本，使用一组新的保守引物对整个编码序列进行测序。结果表明，HBoV最保守的区域是NS1和NP1基因，而VP1和VP2则显示出频繁的变异。然而，完整的编码序列表明，这些变异并不取决于所发现病毒的来源。本研究中确定的完整编码序列可解决HBoV检测方法的问题，该方法可有利地应用于实验室检测。
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