Festy Frederic, Ameer-Beg Simon M, Ng Tony, Suhling Klaus
Randall Division of Cell and Molecular Biophysics, King's College London, London, UK SE1 1UL.
Mol Biosyst. 2007 Jun;3(6):381-91. doi: 10.1039/b617204k. Epub 2007 Mar 26.
Fluorescence lifetime imaging (FLIM) represents a key optical technique for imaging proteins and protein interaction in vivo. We review the principles and recent advances in the application of the technique, instrumentation and molecular probe development.
荧光寿命成像（FLIM）是一种用于在体内对蛋白质和蛋白质相互作用进行成像的关键光学技术。我们综述了该技术的原理、应用、仪器设备及分子探针开发方面的最新进展。
