小牛胸腺DNA聚合酶α催化的单链DNA复制。
Duplication of single stranded DNA catalyzed by calf thymus DNA polymerase alpha.
作者信息
Wang E, Henner D, Furth J J
出版信息
Nucleic Acids Res. 1976 Jan;3(1):129-47. doi: 10.1093/nar/3.1.129.
Purified calf thymus DNA polymerase alpha is inactive with native DNA as template and shows little activity with denatured DNA. DNA synthesis with denatured DNA as template is greatly stimulated by the addition of a nuclease which initially copurifies with DNA polymerase but is separated from the polymerase on DEAE-cellulose chromatography. A limit digest of nuclease treated native DNA which is then denatured is replicated 80-95%; extensive replication is also obtained with native DNA partially degraded by pancreatic DNase and then denatured. The product of the reaction with calf thymus nuclease-treated DNA as template is double-stranded DNA with a hairpin (looped back) structure.
纯化的小牛胸腺DNA聚合酶α以天然DNA为模板时无活性，以变性DNA为模板时活性也很低。以变性DNA为模板的DNA合成可通过添加一种核酸酶得到极大刺激，该核酸酶最初与DNA聚合酶共纯化，但在DEAE - 纤维素色谱上与聚合酶分离。经核酸酶处理的天然DNA经限制性消化后再变性，其复制率为80 - 95%；用胰DNA酶部分降解然后变性的天然DNA也能得到广泛复制。以小牛胸腺核酸酶处理的DNA为模板的反应产物是具有发夹（回环）结构的双链DNA。
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