Qi Shu-Zhen, Wang Qian-Qiu, Tan Yu, Shen Yan, Li Bo, Chen Shu-Li, Cheng Gang, Qin Hong-You, You Zhi-Xue, Zhou Bing-Bin
STD Clinic, Institute of Dermatology, CAMS and PUMC, Nanjing 210042, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2007 Apr;29(2):181-5.
To compare the specificity and sensitivity of two genotyping approaches for human papillomavirus (HPV).
HPV DNA was amplified and detected in clinical specimens by polymerase chain reaction in a pair of universal primers MY09/11, and then genotyped with either sequencing method or liquid chip hybridization method (luminex method).
Sequencing method obtained precise genotyping results in single-type HPV infection, while luminex method obtained accurate genotyping results in multiple-type HPV infection.
A combined method using both sequencing and luminex method is suitable for the genotyping of HPV-infected specimens.
比较两种人乳头瘤病毒（HPV）基因分型方法的特异性和敏感性。
采用一对通用引物MY09/11，通过聚合酶链反应在临床标本中扩增并检测HPV DNA，然后分别采用测序法或液相芯片杂交法（Luminex法）进行基因分型。
测序法在单一型HPV感染中可获得精确的基因分型结果，而Luminex法在多型HPV感染中可获得准确的基因分型结果。
测序法和Luminex法联合使用的方法适用于HPV感染标本的基因分型。
