[Genotyping of genital human papillomavirus by DNA sequencing and luminex methods].

OBJECTIVE

To compare the specificity and sensitivity of two genotyping approaches for human papillomavirus (HPV).

METHOD

HPV DNA was amplified and detected in clinical specimens by polymerase chain reaction in a pair of universal primers MY09/11, and then genotyped with either sequencing method or liquid chip hybridization method (luminex method).

RESULT

Sequencing method obtained precise genotyping results in single-type HPV infection, while luminex method obtained accurate genotyping results in multiple-type HPV infection.

CONCLUSION

A combined method using both sequencing and luminex method is suitable for the genotyping of HPV-infected specimens.