重症肌无力患者血清抗兰尼碱受体抗体的测定及临床意义
[Determination and clinical significance of serum anti-ryanodine receptor antibody in patients with myasthenia gravis].
作者信息
Cui Li-Ying, Li Yan-Feng, Li Yong-Hong, Zhang Jun-Bao
机构信息
Department of Neurology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2007 Apr;29(2):238-40.
OBJECTIVE
To explore the clinical significance of serum anti-ryanodine receptor (RyR) antibody in the diagnosis of myasthenia gravis (MG).
METHODS
The crude sarcoplasmic reticulum was prepared from rabbit skeletal muscle, and then purified by differential centrifugation to produce the antigen. The serum anti-RyR antibody levels in 74 patients with MG (including 21 patients with comorbidic thymomas) were determined with ELISA.
RESULTS
Western blot demonstrated the presence of RyR in purified crude sarcoplasmic reticulum. The positive rate of anti-RyR antibody was significantly higher in MG patients who had comorbidic thymoma compared with those who had no such comorbidity (P < 0.01). Also, the positive rate was closely correlated with the severity of MG.
CONCLUSION
Serum anti-RyR antibody test is helpful in the diagnosis of MG associated with thymoma and can be used to judge the outcome of MG.
目的
探讨血清抗兰尼碱受体（RyR）抗体在重症肌无力（MG）诊断中的临床意义。
方法
从兔骨骼肌制备粗肌浆网，然后通过差速离心进行纯化以制备抗原。采用酶联免疫吸附测定法（ELISA）检测74例MG患者（包括21例合并胸腺瘤患者）的血清抗RyR抗体水平。
结果
蛋白质免疫印迹法显示纯化的粗肌浆网中存在RyR。合并胸腺瘤的MG患者抗RyR抗体阳性率显著高于未合并胸腺瘤的患者（P < 0.01）。此外，阳性率与MG的严重程度密切相关。
结论
血清抗RyR抗体检测有助于胸腺瘤相关MG的诊断，并可用于判断MG的病情。
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