Nash Jason W, Bhardwaj Atul, Wen Ping, Frankel Wendy L
Department of Pathology, The Ohio State University, Columbus, OH 43210-1228, USA.
Appl Immunohistochem Mol Morphol. 2007 Mar;15(1):59-63. doi: 10.1097/01.pai.0000203037.25791.21.
Maspin, a member of the serpin family of serine protease inhibitors, has been shown to limit invasion and metastases in breast and prostate carcinomas. Maspin gene expression is up-regulated in pancreatic cancer, but not in normal pancreatic tissue. Maspin expression has been documented using immunohistochemical studies in pancreatic adenocarcinoma and high-grade intraductal dysplasia. We studied pancreatic ductal adenocarcinomas and chronic pancreatitis utilizing tissue microarray technology to determine the utility of maspin in differentiating these lesions. Immunohistochemistry was performed on tissue microarrays made from 72 cases of pancreatic ductal adenocarcinoma and 24 cases of chronic pancreatitis. Carcinomas were graded as well, moderately, or poorly differentiated using the WHO criteria. The primary antibody used was monoclonal antimaspin antibody (clone G167-70, 1:800, PharMingen, San Diego, CA). Nuclear and/or cytoplasmic staining for maspin was qualitatively scored from 1 + to 3 + based on intensity. Cases were considered positive if one or more cores demonstrated staining. Cases of chronic pancreatitis showed focal, weak (1 + to 2 +) staining within occasional benign ductal epithelial cells in 29% of cases (7/24). Diffuse and intense (3 +) staining was present in ducts with squamous metaplasia (3 cases). The majority of ducts showed no staining. Ductal adenocarcinomas showed diffuse staining in 91% (66/72) of cases with generally more intense staining than cases of chronic pancreatitis. Maspin may be helpful in differentiating ductal adenocarcinoma from chronic pancreatitis, once squamous metaplasia is ruled out.
Maspin是丝氨酸蛋白酶抑制剂丝氨酸蛋白酶抑制剂家族的成员之一，已被证明可限制乳腺癌和前列腺癌的侵袭和转移。Maspin基因在胰腺癌中表达上调，但在正常胰腺组织中不表达。在胰腺腺癌和高级别导管内发育异常中，已通过免疫组织化学研究记录了Maspin的表达。我们利用组织芯片技术研究了胰腺导管腺癌和慢性胰腺炎，以确定Maspin在鉴别这些病变中的作用。对由72例胰腺导管腺癌和24例慢性胰腺炎制成的组织芯片进行了免疫组织化学分析。根据世界卫生组织标准，将癌分为高分化、中分化或低分化。使用的一抗是单克隆抗Maspin抗体（克隆G167 - 70，1:800，Pharmingen，圣地亚哥，加利福尼亚州）。根据强度，对Maspin的核和/或细胞质染色进行定性评分，从1 +到3 +。如果一个或多个核心显示染色，则病例被认为是阳性。29%（7/24）的慢性胰腺炎病例在偶尔的良性导管上皮细胞内显示局灶性、弱阳性（1 +至2 +）染色。在有鳞状化生的导管中存在弥漫性强染色（3 +）（3例）。大多数导管未显示染色。91%（66/72）的导管腺癌病例显示弥漫性染色，总体染色强度通常比慢性胰腺炎病例更强。一旦排除鳞状化生，Maspin可能有助于区分导管腺癌和慢性胰腺炎。
