Bangerter Markus, Brudler Olaf, Heinrich Bernhard, Griesshamnuer Martin
Hematological and Oncological Practice, Augsburg, Germany.
Acta Cytol. 2007 May-Jun;51(3):390-8. doi: 10.1159/000325753.
To determine the usefulness of fine needle aspiration cytology (FNAC) in combination with flow cytometry on the new World Health Organization (WHO) classification of malignant lymphoma.
Smears and flow cytometry reports of patients who underwent both methods at the same time were independently examined. Both methods were classified according to the new WHO classification of malignant lymphoma.
A group of 131 smears were examined. In 89 cases exact diagnosis was made by cytomorphology. Twenty-five cases were not classified exactly or were classified incorrectly, resulting in a sensitivity of 96.4% and a specificity of 85%. With flow cytometry, only 30 of 131 patients could be classified exactly, resulting in a sensitivity of 27% and specificity of 100%, respectively. The combination of methods showed a sensitivity of 85% and specificity of 100%.
The combination of FNAC and flow cytometry obtained by FNAC can distinguish between benign and malignant lymphoid infiltrates and support a diagnosis of lymphoma.
确定细针穿刺细胞学检查（FNAC）联合流式细胞术在世界卫生组织（WHO）恶性淋巴瘤新分类中的应用价值。
对同时接受这两种检查方法的患者的涂片和流式细胞术报告进行独立检查。两种方法均根据WHO恶性淋巴瘤新分类进行分类。
共检查了131份涂片。89例通过细胞形态学做出了准确诊断。25例未得到准确分类或分类错误，敏感性为96.4%，特异性为85%。流式细胞术方面，131例患者中仅30例能得到准确分类，敏感性分别为27%，特异性为100%。两种方法联合使用时，敏感性为85%，特异性为100%。
FNAC联合通过FNAC获得的流式细胞术能够区分良性和恶性淋巴浸润，并辅助淋巴瘤的诊断。
