Steuhl K P, Rohrbach J M, Knorr M
Universitäts-Augenklinik Tübingen, Abteilung I.
Klin Monbl Augenheilkd. 1991 Sep;199(3):187-91. doi: 10.1055/s-2008-1046069.
The reactivity of the monoclonal antibody HMB 45 was evaluated in melanocytic tumors of the conjunctiva. Among these are 10 acquired melanoses, 19 nevi and 34 melanomas. Results were compared with the presence of the S 100 antigen. Especially the intraepithelial and junctional components of primarily benign lesions were stained with HMB 45. Within malignant melanoma this antibody reacts with melanocytes in the epithelial, junctional and subepithelial areas. The polyclonal antibody S 100 stains all melanocytes in pigmented lesions of the conjunctiva. Intraepithelial or subepithelial malignant infiltrating tumor cells show very intense staining with HMB 45. HMB 45 has therefore high specificity for stimulated melanocytes, but it does not distinguish benign and malignant proliferating melanocytic cells.
对单克隆抗体HMB 45在结膜黑素细胞肿瘤中的反应性进行了评估。其中包括10例获得性黑素沉着、19例痣和34例黑色素瘤。将结果与S 100抗原的存在情况进行了比较。尤其是原发性良性病变的上皮内和交界成分被HMB 45染色。在恶性黑色素瘤中，该抗体与上皮、交界和上皮下区域的黑素细胞发生反应。多克隆抗体S 100可对结膜色素沉着病变中的所有黑素细胞进行染色。上皮内或上皮下恶性浸润性肿瘤细胞用HMB 45染色显示非常强烈。因此，HMB 45对受刺激的黑素细胞具有高度特异性，但它不能区分良性和恶性增殖的黑素细胞。
