Pathak Smita, Davidson Marie C, Silva Gabriel A
Materials Science and Engineering Graduate Program, Department of Bioengineering, University of California, San Diego, La Jolla, California 92037-0946, USA.
Nano Lett. 2007 Jul;7(7):1839-45. doi: 10.1021/nl062706i. Epub 2007 May 31.
Antibody conjugated quantum dots are an emerging technology for high-resolution labeling of biological systems. In this work we determined the number of functional antibodies (i.e., antibodies that are sterically available for functional binding to target proteins) conjugated to semiconductor quantum dots. This is critical for the interpretation of biological data labeled with these methods. We found that the number of available functional antibodies varied significantly for different conjugation methods and are lower than previously estimated. These results may suggest potential strategies for improving quantum dot labeling of biological preparations.
抗体偶联量子点是一种用于生物系统高分辨率标记的新兴技术。在这项工作中，我们确定了与半导体量子点偶联的功能性抗体（即空间上可用于与靶蛋白进行功能性结合的抗体）的数量。这对于解释用这些方法标记的生物学数据至关重要。我们发现，不同偶联方法的可用功能性抗体数量差异显著，且低于先前的估计。这些结果可能为改进生物制剂的量子点标记提供潜在策略。
