Singh Gurpal, Zaidi Neelam Hazoor, Soni Udit, Gautam Manoj, Jackeray Richa, Singh Harpal, Sapra Sameer
Centre for Biomedical Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India.
J Nanosci Nanotechnol. 2011 May;11(5):3834-42. doi: 10.1166/jnn.2011.4169.
Bioconjugation of quantum dots has resulted in a significant increase in resolution of biological fluorescent labeling. This intrinsic property of quantum dots can be utilized for sensitive detection of target analytes with high sensitivity; including pathogenic bacteria and cancer monitoring. The quantum dots and quantum dot doped silica nanoparticles exhibit prominent emission peaks when excited at 400 nm but on conjugation to model rabbit antigoat antibodies exhibit diminished intensity of emission peak at 600 nm. It shows that photoluminescence intensity of conjugated quantum dots and quantum dot doped silica nanoparticles could permit the detection of bioconjugation. Samples of conjugated and unconjugated quantum dots and quantum dot doped silica nanoparticles were subjected to enzyme linked immunosorbent assay for further confirmation of bioconjugation. In the present study ligand exchange, bioconjugation, fluorescence detection of bioconjugated quantum dots and quantum dot doped silica nanoparticles and further confirmation of bioconjugation by enzyme linked immunosorbent assay has been described.
量子点的生物共轭作用显著提高了生物荧光标记的分辨率。量子点的这种固有特性可用于高灵敏度地检测目标分析物，包括病原菌和癌症监测。量子点和掺杂量子点的二氧化硅纳米颗粒在400nm激发时呈现出明显的发射峰，但与兔抗山羊抗体模型共轭后，600nm处的发射峰强度减弱。这表明共轭量子点和掺杂量子点的二氧化硅纳米颗粒的光致发光强度可用于检测生物共轭作用。对共轭和未共轭的量子点以及掺杂量子点的二氧化硅纳米颗粒样品进行酶联免疫吸附测定，以进一步确认生物共轭作用。在本研究中，描述了配体交换、生物共轭、共轭量子点和掺杂量子点的二氧化硅纳米颗粒的荧光检测以及通过酶联免疫吸附测定对生物共轭作用的进一步确认。
