环磷腺苷（cAMP）在体外对垂体前叶激素释放的影响。

The involvement of cyclic-3',5'-AMP in the release of hormones from the anterior pituitary in vitro.

作者信息

Sundberg D K, Fawcett C P, McCann S M

出版信息

Proc Soc Exp Biol Med. 1976 Jan;151(1):149-54. doi: 10.3181/00379727-151-39163.

Abstract

DBcAMP significantly increased the release of GH but not of LH, FSH, TSH, or PRL, except in the presence of hypothalamic extract when it augmented the release of LH, FSH, and GH, reversed the inhibition of PRL, but did not further influence TSH release. Theophylline increased release of GH and PRL while inducing increased tissue content of cAMP without consistently increasing the release of TSH, LH, or FSH. Hypothalamic extractor K+-stimulated hormone rel-ase was consistently and significantly potentiated by theophylline. Neither hypothalamic extract, increased [K+], or synthetic TRH and LRH were able to raise tissue content of cAMP while producing their expected effects on hormone release. Cholera enterotoxin produced a highly significant increase in tissue content of the cyclic nucleotide but increased the release of GH only, and not that of LH, FSH, TSH, or PRL. DBcAMP was able to lower the threshold concentration of K+ required to stimulate release of GH, LH, and FSH and also to augment K+-stimulated release to the higher levels induced by the hypothalamic releasing hormones. It did not augment K+-induced release of TSH.

摘要

二丁酰环磷腺苷（DBcAMP）显著增加了生长激素（GH）的释放，但对促黄体生成素（LH）、促卵泡生成素（FSH）、促甲状腺激素（TSH）或催乳素（PRL）的释放没有影响，不过在下丘脑提取物存在的情况下，它增加了LH、FSH和GH的释放，逆转了对PRL的抑制作用，但对TSH的释放没有进一步影响。茶碱增加了GH和PRL的释放，同时诱导细胞内cAMP含量增加，但对TSH、LH或FSH的释放没有持续增加。下丘脑提取物经钾离子（K⁺）刺激后的激素释放被茶碱持续且显著地增强。下丘脑提取物、升高的[K⁺]、合成的促甲状腺激素释放激素（TRH）和促性腺激素释放激素（LRH）在对激素释放产生预期作用时，均不能提高细胞内cAMP含量。霍乱肠毒素使环核苷酸的细胞内含量显著增加，但仅增加了GH的释放，对LH、FSH、TSH或PRL的释放没有影响。DBcAMP能够降低刺激GH、LH和FSH释放所需的K⁺阈值浓度，还能将K⁺刺激的释放提高到下丘脑释放激素诱导的更高水平。它没有增强K⁺诱导的TSH释放。