下丘脑释放激素对垂体单层培养物中促黄体生成素、促卵泡激素和催乳素的影响。

Effects of hypothalamic-releasing hormones on LH, FSH, and prolactin in pituitary monolayer cultures.

作者信息

Tang L K, Spies H G

出版信息

Proc Soc Exp Biol Med. 1976 Jan;151(1):189-92. doi: 10.3181/00379727-151-39171.

Abstract

Four-day-old pituitary monolayer cultures were incubated with various hypothalamic releasing hormones. Rat hypothalamic extract stimulated the release of LH, FSH, and PRL by these cultures in a dose-related fashion. Synthetic LH-RH stimulated the release of LH and FSH but not of PRL. Synthetic TRH increased the release of PRL but had no effect on LH or FSH. At 10(-8) M, somatostatin did not affect any of the three adenohypophyseal hormones. Incubation with DBcAMP or theophylline also stimulated PRL release without any detectable effect on LH and FSH release. These data suggest the involvement of cyclic AMP--adenylate cyclase system in the mechanism of PRL release, but their involvement in gonadotropin release requires further studies.

摘要

将4日龄的垂体单层培养物与各种下丘脑释放激素一起孵育。大鼠下丘脑提取物以剂量相关的方式刺激这些培养物释放促黄体生成素（LH）、促卵泡生成素（FSH）和催乳素（PRL）。合成的促黄体生成素释放激素（LH-RH）刺激LH和FSH的释放，但不刺激PRL的释放。合成的促甲状腺激素释放激素（TRH）增加PRL的释放，但对LH或FSH没有影响。在10^(-8) M时，生长抑素对三种腺垂体激素中的任何一种都没有影响。与二丁酰环磷腺苷（DBcAMP）或茶碱一起孵育也刺激PRL释放，而对LH和FSH释放没有任何可检测到的影响。这些数据表明环磷酸腺苷-腺苷酸环化酶系统参与PRL释放机制，但其在促性腺激素释放中的作用需要进一步研究。