Lloyd R V, McShan W H
Proc Soc Exp Biol Med. 1976 Jan;151(1):160-2. doi: 10.3181/00379727-151-39165.
Rat anterior pituitary glands were dissociated with Pronase and the cells were separated by velocity sedimentation at unit gravity. After 30 min of incubation of the enriched gonadotropic cells with LH-RH, there was a significant increase in LH and FSH in the incubation medium. LH-RH (100 ng/ml) and 10(-3) M cAMP both caused significant increases in LH in the incubation medium after 24 hr of incubation.
用链霉蛋白酶解离大鼠垂体前叶，通过单位重力下的速率沉降分离细胞。将富集的促性腺细胞与促黄体生成素释放激素（LH-RH）孵育30分钟后，孵育培养基中LH和FSH显著增加。孵育24小时后，LH-RH（100纳克/毫升）和10⁻³摩尔/升环磷酸腺苷（cAMP）均使孵育培养基中的LH显著增加。
