Roberts E D, Martin L N
Delta Regional Primate Research Center, Tulane University, Covington, Louisiana.
Lab Invest. 1991 Dec;65(6):637-43.
The joint tissues from 36 rhesus (age 2-5 years) infected with SIV/Delta were graded into four groups and then correlated with the following systemic immunologic parameters: numbers of CD4+, CD4+ plus CD29+, and CD20+ cells and SIV/Delta antigenemia. Grade I (n = 16) consisted of normal synovial membrane; grade II (n = 8) consisted of synovial hyperplasia; grade III (n = 8) consisted of a mononuclear cell infiltrate, and grade IV (n = 4) consisted of synovial membrane infiltration of syncytial cell and mononuclear type cells. Synovial membranes from 20 uninfected age-matched rhesus were compared with infected animals and were graded 1. Immunostaining of the tissue phase lymphocytes and viral antigen was performed using monoclonal antibodies commercially available and a monoclonal antibody produced against a core protein of the SIV/Delta agent. SIV/Delta infection resulted in a reduction in cells doubly stained for CD4+ and CD29+ and a relative increase in CD8+ stained cells. A significant (ANOVA) difference was not observed in CD4+, CD4+ plus CD29+, CD8+, or CD20+ among the various grades of arthritis. Viral antigen was demonstrated in syncytial cells of the grade IV reaction. This finding suggests that the SIV/Delta arthritis is a primary viral reaction.
将36只感染SIV/Delta的恒河猴（年龄2 - 5岁）的关节组织分为四组，然后与以下全身免疫参数相关联：CD4⁺、CD4⁺加CD29⁺以及CD20⁺细胞的数量和SIV/Delta抗原血症。I级（n = 16）由正常滑膜组成；II级（n = 8）由滑膜增生组成；III级（n = 8）由单核细胞浸润组成，IV级（n = 4）由合胞体细胞和单核细胞类型细胞的滑膜浸润组成。将20只未感染的年龄匹配恒河猴的滑膜与感染动物的滑膜进行比较，并分级为1级。使用市售单克隆抗体和针对SIV/Delta制剂核心蛋白产生的单克隆抗体对组织期淋巴细胞和病毒抗原进行免疫染色。SIV/Delta感染导致CD4⁺和CD29⁺双染细胞减少，CD8⁺染色细胞相对增加。在不同等级的关节炎中，CD4⁺、CD4⁺加CD29⁺、CD8⁺或CD20⁺之间未观察到显著（方差分析）差异。在IV级反应的合胞体细胞中检测到病毒抗原。这一发现表明SIV/Delta关节炎是一种原发性病毒反应。
