[同型半胱氨酸降低培养的人滋养层细胞的侵袭能力：同型半胱氨酸与基质金属蛋白酶-2、-9表达之间的关系]
[Homocysteine decreases the invasion in cultured human trophoblasts: relationship between homocysteine and matrix metalloproteinase-2, -9 expression].
作者信息
Yang Pei-feng, Li Jin-ke, Xiong Qing
机构信息
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Zhonghua Fu Chan Ke Za Zhi. 2007 Mar;42(3):184-6.
OBJECTIVE
To test the hypothesis that homocysteine can decrease MMP-2 and MMP-9 expression in cultured trophoblasts of early pregnancy and that homocysteine can prevent trophoblasts invasion in the early stage of preeclampsia.
METHODS
Cytotrophoblasts from early pregnancy were isolated and cultured. Trophoblasts were treated with or without Hcy (1 mmol/L) for 48 hour, and real time RT-PCR and gelatin zymography were used to quantify the mRNA and protease activity of MMP-2, -9.
RESULTS
Treatment with Hcy (1 mmol/L) induced a decrease in MMP-2 mRNA by 21% and MMP-9 mRNA by 11%. At protein level MMP-2 expression decreased 14% and MMP-9 expression decreased 52% compared with control.
CONCLUSIONS
Homocysteine can decrease MMP-2, -9 expression in trophoblasts of early pregnancy and influence its invasion process.
目的
验证同型半胱氨酸可降低早孕滋养层细胞中基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）的表达，以及同型半胱氨酸可在子痫前期早期阻止滋养层细胞侵袭这一假说。
方法
分离并培养早孕细胞滋养层细胞。用或不用同型半胱氨酸（1毫摩尔/升）处理滋养层细胞48小时，采用实时逆转录聚合酶链反应（RT-PCR）和明胶酶谱法对MMP-2、MMP-9的信使核糖核酸（mRNA）和蛋白酶活性进行定量分析。
结果
用同型半胱氨酸（1毫摩尔/升）处理后，MMP-2的mRNA降低了21%，MMP-9的mRNA降低了11%。与对照组相比，在蛋白质水平上，MMP-2的表达降低了14%，MMP-9的表达降低了52%。
结论
同型半胱氨酸可降低早孕滋养层细胞中MMP-2、MMP-9的表达，并影响其侵袭过程。
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