通过摄取血液诱导蚊子中肠肌动蛋白基因表达与细胞形状的显著变化相关。
Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape.
作者信息
Sodja Ann, Fujioka Hisashi, Lemos Francisco J A, Donnelly-Doman Marilyn, Jacobs-Lorena Marcelo
机构信息
Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA.
出版信息
J Insect Physiol. 2007 Aug;53(8):833-9. doi: 10.1016/j.jinsphys.2007.03.017. Epub 2007 Apr 19.
Abstract
Ingestion of a blood meal by the female mosquito Anopheles gambiae (L., Diptera: Culicidae), results in a dramatic distention of the midgut epithelium. Here, we report that these events correlate with a transient increase of actin mRNA and protein abundance. The newly synthesized actin may provide a pool of actin protein needed to remodel epithelial cell cytoarchitecture. We also document changes in midgut epithelial cell morphology. Upon blood ingestion, the columnar cells flatten accompanied by the loss of microvilli on the lumenal side and the unfolding of the labyrinth on the basal side. These changes correlate with the large increase of epithelial surface area needed to accommodate the blood meal. Actin gene expression, actin synthesis and cell morphology all return to the pre-feeding state by 24 h after blood intake.
摘要
冈比亚按蚊（双翅目：蚊科）雌蚊摄取血餐会导致中肠上皮显著扩张。在此，我们报告这些事件与肌动蛋白mRNA和蛋白质丰度的短暂增加相关。新合成的肌动蛋白可能提供重塑上皮细胞细胞结构所需的肌动蛋白蛋白库。我们还记录了中肠上皮细胞形态的变化。摄取血餐时，柱状细胞变平，同时腔侧微绒毛丧失，基底侧迷路展开。这些变化与容纳血餐所需的上皮表面积大幅增加相关。摄取血餐24小时后，肌动蛋白基因表达、肌动蛋白合成和细胞形态均恢复到进食前状态。
相似文献
1
Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape.通过摄取血液诱导蚊子中肠肌动蛋白基因表达与细胞形状的显著变化相关。
J Insect Physiol. 2007 Aug;53(8):833-9. doi: 10.1016/j.jinsphys.2007.03.017. Epub 2007 Apr 19.
2
Storage and secretion of the peritrophic matrix protein Ag-Aper1 and trypsin in the midgut of Anopheles gambiae.冈比亚按蚊中肠围食膜蛋白Ag-Aper1和胰蛋白酶的储存与分泌
Insect Mol Biol. 2004 Aug;13(4):349-58. doi: 10.1111/j.0962-1075.2004.00488.x.
3
The Midgut Muscle Network of Anopheles aquasalis (Culicidae, Anophelinae): Microanatomy and Structural Modification After Blood Meal and Plasmodium vivax (Haemosporida, Plasmodiidae) Infection.《嗜人按蚊（蚊科，按蚊亚科）中肠肌网络：血餐和间日疟原虫（血孢子虫目，疟原虫科）感染后的微观解剖和结构修饰》
J Med Entomol. 2019 Feb 25;56(2):421-431. doi: 10.1093/jme/tjy199.
4
Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding.疟疾传播媒介白纹按蚊吸血后早期中肠的形态学和蛋白质组学特征
J Proteomics. 2014 Dec 5;111:100-12. doi: 10.1016/j.jprot.2014.07.037. Epub 2014 Aug 13.
5
Midgut morphology of the predator mosquito Lutzia bigoti (Diptera: Culicidae) and its implications for feeding behavior.捕食性蚊种大蚊长管亚种（双翅目：蚊科）中肠形态及其对取食行为的影响。
Acta Trop. 2024 Sep;257:107289. doi: 10.1016/j.actatropica.2024.107289. Epub 2024 Jun 13.
6
Fz2 and cdc42 mediate melanization and actin polymerization but are dispensable for Plasmodium killing in the mosquito midgut.Fz2和cdc42介导黑化作用和肌动蛋白聚合，但对于疟原虫在蚊子中肠中的杀灭是可有可无的。
PLoS Pathog. 2006 Dec;2(12):e133. doi: 10.1371/journal.ppat.0020133.
7
CNAct-1 gene is differentially expressed in the subtropical mosquito Culex nigripalpus (Diptera: Culicidae), the primary West Nile Virus vector in Florida.
J Med Entomol. 2008 Sep;45(5):877-84. doi: 10.1603/0022-2585(2008)45[877:cgidei]2.0.co;2.
8
Translational regulation of Anopheles gambiae mRNAs in the midgut during Plasmodium falciparum infection.疟原虫感染期间按蚊中肠 mRNA 的翻译调控。
BMC Genomics. 2012 Aug 2;13:366. doi: 10.1186/1471-2164-13-366.
9
Blood digestion in the mosquito, Anopheles stephensi Liston (Diptera: Culicidae): activity and distribution of trypsin, aminopeptidase, and alpha-glucosidase in the midgut.斯氏按蚊（双翅目：蚊科）中血液的消化：中肠内胰蛋白酶、氨肽酶和α-葡萄糖苷酶的活性及分布
J Med Entomol. 1991 Nov;28(6):865-71. doi: 10.1093/jmedent/28.6.865.
10
Additional Feeding Reveals Differences in Immune Recognition and Growth of Parasites in the Mosquito Host.额外喂养揭示了蚊子宿主中寄生虫的免疫识别和生长的差异。
mSphere. 2021 Mar 31;6(2):e00136-21. doi: 10.1128/mSphere.00136-21.
引用本文的文献
1
Glycosylation Patterns in Gut: Implications for the Development of Vector Control Strategies.肠道中的糖基化模式：对病媒控制策略发展的启示
Microorganisms. 2025 Jan 1;13(1):58. doi: 10.3390/microorganisms13010058.
2
Anopheles coluzzii stearoyl-CoA desaturase is essential for adult female survival and reproduction upon blood feeding.按蚊酰基辅酶 A 去饱和酶对成蚊雌性吸血后的存活和繁殖是必需的。
PLoS Pathog. 2021 May 20;17(5):e1009486. doi: 10.1371/journal.ppat.1009486. eCollection 2021 May.
3
Additional Feeding Reveals Differences in Immune Recognition and Growth of Parasites in the Mosquito Host.额外喂养揭示了蚊子宿主中寄生虫的免疫识别和生长的差异。
mSphere. 2021 Mar 31;6(2):e00136-21. doi: 10.1128/mSphere.00136-21.
4
An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans.角蝇唾液组、肌浆组和病毒组的深入研究。
BMC Genomics. 2019 Jul 29;20(1):616. doi: 10.1186/s12864-019-5984-7.
5
Microanatomy of the American Malaria Vector Anopheles aquasalis (Diptera: Culicidae: Anophelinae) Midgut: Ultrastructural and Histochemical Observations.美洲疟蚊按蚊属（双翅目：蚊科：按蚊亚科）中肠的微观解剖：超微结构和组织化学观察。
J Med Entomol. 2019 Oct 28;56(6):1636-1649. doi: 10.1093/jme/tjz114.
6
Identification of new protein interactions between dengue fever virus and its hosts, human and mosquito.鉴定登革热病毒与其宿主（人类和蚊子）之间的新蛋白相互作用。
PLoS One. 2013;8(1):e53535. doi: 10.1371/journal.pone.0053535. Epub 2013 Jan 11.
7
The interplay between tubulins and P450 cytochromes during Plasmodium berghei invasion of Anopheles gambiae midgut.疟原虫入侵冈比亚按蚊中肠过程中微管蛋白与 P450 细胞色素的相互作用。
PLoS One. 2011;6(8):e24181. doi: 10.1371/journal.pone.0024181. Epub 2011 Aug 30.
8
Defects in coatomer protein I (COPI) transport cause blood feeding-induced mortality in Yellow Fever mosquitoes.衣壳蛋白 I（COPI）运输缺陷导致黄热病蚊子在吸血后死亡。
Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):E211-7. doi: 10.1073/pnas.1102637108. Epub 2011 May 31.
9
Anopheles aquasalis Infected by Plasmodium vivax displays unique gene expression profiles when compared to other malaria vectors and plasmodia.按蚊aquasalis 感染疟原虫vivax 时的基因表达谱与其他疟疾媒介和疟原虫相比具有独特性。
PLoS One. 2010 Mar 22;5(3):e9795. doi: 10.1371/journal.pone.0009795.
10
Localisation of laminin within Plasmodium berghei oocysts and the midgut epithelial cells of Anopheles stephensi.疟原虫孢子和斯蒂芬斯按蚊中肠上皮细胞中层粘连蛋白的定位。
Parasit Vectors. 2008 Sep 22;1(1):33. doi: 10.1186/1756-3305-1-33.
本文引用的文献
1
Upregulation of two actin genes and redistribution of actin during diapause and cold stress in the northern house mosquito, Culex pipiens.北方家蚊库蚊滞育和冷应激期间两个肌动蛋白基因的上调及肌动蛋白的重新分布
J Insect Physiol. 2006 Nov-Dec;52(11-12):1226-33. doi: 10.1016/j.jinsphys.2006.09.007. Epub 2006 Sep 20.
2
Genome-wide analysis of gene expression in adult Anopheles gambiae.冈比亚按蚊成虫基因表达的全基因组分析。
Insect Mol Biol. 2006 Feb;15(1):1-12. doi: 10.1111/j.1365-2583.2006.00610.x.
3
Sequence and comparative genomic analysis of actin-related proteins.肌动蛋白相关蛋白的序列及比较基因组分析
Mol Biol Cell. 2005 Dec;16(12):5736-48. doi: 10.1091/mbc.e05-06-0508. Epub 2005 Sep 29.
4
The global distribution of clinical episodes of Plasmodium falciparum malaria.恶性疟原虫疟疾临床发作的全球分布情况。
Nature. 2005 Mar 10;434(7030):214-7. doi: 10.1038/nature03342.
5
Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae.与冈比亚疟蚊吸血相关的基因表达模式。
BMC Genomics. 2005 Jan 14;6:5. doi: 10.1186/1471-2164-6-5.
6
Storage and secretion of the peritrophic matrix protein Ag-Aper1 and trypsin in the midgut of Anopheles gambiae.冈比亚按蚊中肠围食膜蛋白Ag-Aper1和胰蛋白酶的储存与分泌
Insect Mol Biol. 2004 Aug;13(4):349-58. doi: 10.1111/j.0962-1075.2004.00488.x.
7
Identification of functionally important residues of Arp2/3 complex by analysis of homology models from diverse species.通过分析来自不同物种的同源模型鉴定Arp2/3复合物的功能重要残基。
J Mol Biol. 2004 Feb 13;336(2):551-65. doi: 10.1016/j.jmb.2003.12.017.
8
A molecular view of trypsin synthesis in the midgut of Aedes aegypti.埃及伊蚊中肠胰蛋白酶合成的分子视角。
J Insect Physiol. 1999 Jul;45(7):613-620. doi: 10.1016/s0022-1910(99)00052-9.
9
The genome sequence of the malaria mosquito Anopheles gambiae.冈比亚按蚊——疟蚊的基因组序列。
Science. 2002 Oct 4;298(5591):129-49. doi: 10.1126/science.1076181.
10
Functional morphology of adult female Culex quinquefasciatus midgut during blood digestion.
Tissue Cell. 2002 Jun;34(3):210-9. doi: 10.1016/s0040-8166(02)00032-0.
Zotero 插件