通过摄取血液诱导蚊子中肠肌动蛋白基因表达与细胞形状的显著变化相关。
Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape.
作者信息
Sodja Ann, Fujioka Hisashi, Lemos Francisco J A, Donnelly-Doman Marilyn, Jacobs-Lorena Marcelo
机构信息
Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA.
出版信息
J Insect Physiol. 2007 Aug;53(8):833-9. doi: 10.1016/j.jinsphys.2007.03.017. Epub 2007 Apr 19.
Abstract
Ingestion of a blood meal by the female mosquito Anopheles gambiae (L., Diptera: Culicidae), results in a dramatic distention of the midgut epithelium. Here, we report that these events correlate with a transient increase of actin mRNA and protein abundance. The newly synthesized actin may provide a pool of actin protein needed to remodel epithelial cell cytoarchitecture. We also document changes in midgut epithelial cell morphology. Upon blood ingestion, the columnar cells flatten accompanied by the loss of microvilli on the lumenal side and the unfolding of the labyrinth on the basal side. These changes correlate with the large increase of epithelial surface area needed to accommodate the blood meal. Actin gene expression, actin synthesis and cell morphology all return to the pre-feeding state by 24 h after blood intake.
摘要
冈比亚按蚊（双翅目：蚊科）雌蚊摄取血餐会导致中肠上皮显著扩张。在此，我们报告这些事件与肌动蛋白mRNA和蛋白质丰度的短暂增加相关。新合成的肌动蛋白可能提供重塑上皮细胞细胞结构所需的肌动蛋白蛋白库。我们还记录了中肠上皮细胞形态的变化。摄取血餐时，柱状细胞变平，同时腔侧微绒毛丧失，基底侧迷路展开。这些变化与容纳血餐所需的上皮表面积大幅增加相关。摄取血餐24小时后，肌动蛋白基因表达、肌动蛋白合成和细胞形态均恢复到进食前状态。
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