Li Qiang, Xing Da, Jia Li, Wang Junsheng
MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China.
J Photochem Photobiol B. 2007 Jun 26;87(3):183-90. doi: 10.1016/j.jphotobiol.2007.04.005. Epub 2007 Apr 24.
A mathematical-physical analysis model, which describes individually the electronic reflux of several significant components in the photosynthesis electron transport chain, was firstly developed. The process of electrons flowing back to the oxidized reaction center P(680)(+) was simulated by a series of photochemical reaction equations, resulting in getting the linked differential equations of delayed fluorescence (DF) intensity. MATLAB provided a computationally efficient method to solve these linked equations. Simulations based on this model showed that the decay kinetics of DF accord with double exponential. DF components decaying in the millisecond range (fast phase) are related to the charge recombination of P(680)(+) and Q(A)(-). The components decaying in the seconds range are associated with the recombination of P(680)(+) with Q(B)(2-). The developed model was tested in maize leaves treated with different electron blockers to induce changes in photosynthesis electron transport chain. The experimental results demonstrated that the developed model can accurately determine the regulatory effects of electron blockers on photosynthesis electron transport chain. Therefore, the model presented here could be potentially useful for studying the electron transfer in plant. It also provides an experimental workbench for testing hypotheses as to the underlying mechanism controlling the change for different phases of DF.
首次建立了一个数学物理分析模型，该模型分别描述了光合作用电子传递链中几个重要组分的电子回流。通过一系列光化学反应方程模拟电子回流到氧化反应中心P(680)(+)的过程，得到了延迟荧光（DF）强度的联立微分方程。MATLAB提供了一种计算效率高的方法来求解这些联立方程。基于该模型的模拟表明，DF的衰减动力学符合双指数规律。在毫秒范围内衰减的DF组分（快相）与P(680)(+)和Q(A)(-)的电荷复合有关。在秒范围内衰减的组分与P(680)(+)与Q(B)(2-)的复合有关。用不同的电子阻断剂处理玉米叶片以诱导光合作用电子传递链发生变化，对所建立的模型进行了测试。实验结果表明，所建立的模型能够准确地确定电子阻断剂对光合作用电子传递链的调节作用。因此，本文提出的模型可能对研究植物中的电子传递有用。它还为检验关于控制DF不同阶段变化的潜在机制的假设提供了一个实验平台。
