Becker Timothy A, Preul Mark C, Bichard William D, Kipke Daryl R, McDougall Cameron G
Neural Engineering Laboratory, Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Neurosurgery. 2007 Jun;60(6):1119-27; discussion 1127-8. doi: 10.1227/01.NEU.0000255447.90106.12.
We sought to expand our assessment of calcium alginate as an embolic agent in an aneurysm model in swine that survived from 30 to 90 days. The objective of this study was to assess the biocompatibility and stability of calcium alginate in aneurysms in vivo.
Ten models were created from a venous pouch sutured to the carotid artery, simulating flow to a side-wall aneurysm. Eight swine received complete embolizations, and two were less than 50% embolized to be used as controls. Alginate and calcium chloride were injected from concentric-tube microcatheters to form a mass that filled the aneurysm pouch.
Angiography and histology verified complete aneurysm occlusion and neck healing up to 90 days in eight swine. Both control animal aneurysms ruptured within 8 days. No animals showed evidence of downstream calcium alginate gel propagation. A minor bioactive response to the alginate gel was noted at 30 days, and fibrous tissue grew over the aneurysm orifice, sealing off the defect. No degenerative or inflammatory response was observed. At 90 days, moderate fibrous tissue surrounded the alginate. Tissue growth across the aneurysm neck remained complete and stable with no signs of neointimal growth into the parent vessel.
Calcium alginate was an effective endovascular occlusion material that filled the aneurysm and provided an effective template for tissue growth across the aneurysm neck after 30 days and up to 90 days. Complete filling of the aneurysm with calcium alginate ensures stability, biocompatibility, and optimal healing for up to 90 days in swine.
我们试图在存活30至90天的猪动脉瘤模型中扩大对海藻酸钙作为栓塞剂的评估。本研究的目的是评估海藻酸钙在体内动脉瘤中的生物相容性和稳定性。
通过将静脉囊缝合到颈动脉上创建10个模型，模拟侧壁动脉瘤的血流。8只猪接受了完全栓塞，2只栓塞少于50%作为对照。从同心管微导管注射海藻酸盐和氯化钙，形成填充动脉瘤囊的团块。
血管造影和组织学证实8只猪的动脉瘤完全闭塞且颈部愈合长达90天。两只对照动物的动脉瘤在8天内破裂。没有动物显示出海藻酸钙凝胶向远端传播的迹象。在30天时观察到对海藻酸钙凝胶有轻微的生物活性反应，纤维组织在动脉瘤口生长，封闭了缺损。未观察到退行性或炎症反应。在90天时，海藻酸钙周围有中等程度的纤维组织。动脉瘤颈部的组织生长保持完整和稳定，没有新生内膜长入母血管的迹象。
海藻酸钙是一种有效的血管内闭塞材料，可填充动脉瘤，并在30天至90天内为动脉瘤颈部的组织生长提供有效的模板。在猪体内，用海藻酸钙完全填充动脉瘤可确保长达90天的稳定性、生物相容性和最佳愈合。
