在小动物正电子发射断层扫描（PET）研究中，眼眶后注射是在小鼠体内给药放射性药物的一种有效途径。

Retro-orbital injection is an effective route for radiopharmaceutical administration in mice during small-animal PET studies.

作者信息

Nanni Cristina, Pettinato Cinzia, Ambrosini Valentina, Spinelli Antonello, Trespidi Silvia, Rubello Domenico, Al-Nahhas Adil, Franchi Roberto, Alavi Abass, Fanti Stefano

机构信息

Nuclear Medicine Department, Azienda Ospedaliero-Universitaria di Bologna Policlinico S. Orsola-Malpighi, Italy.

出版信息

Nucl Med Commun. 2007 Jul;28(7):547-53. doi: 10.1097/MNM.0b013e3281fbd42b.

DOI:10.1097/MNM.0b013e3281fbd42b

PMID:17538396

Abstract

BACKGROUND AND AIM

Small-animal PET is acquiring importance for pre-clinical studies. In rodents, radiotracers are usually administrated via the tail vein. This procedure can be very difficult and time-consuming as soft tissue extravasations are very frequent and tail scars can prevent repeated injections after initial failure. The aim of our study was to compare the retro-orbital (RO) versus tail vein intravenous (i.v.) administration of (18)F-FDG and (11)C-choline in mice for small-animal PET studies.

METHODS

We evaluated four healthy female ICR CD1 mice according to the following protocol. Day 1: each animal underwent an i.v. injection of 28 MBq of (11)C-choline. PET scan was performed after 10 min and 40 min. Day 2: each animal received an RO injection of 28 MBq of (11)C-choline. A PET scan was performed after 10 min and 40 min. Day 3: each animal received an i.v. injection of 28 MBq of (18)F-FDG. A PET scan was performed after 60 min and 120 min. Day 4: each animal received an RO injection of 28 MBq of (18)F-FDG. A PET scan was performed after 60 min and 120 min. Administration and image acquisition were performed under gas anaesthesia. For FDG studies the animals fasted for 2 h and were kept asleep for 20-30 min after injection, to avoid muscular uptake. Images were reconstructed with 2-D OSEM. For each scan ROIs were drawn on liver, kidneys, lung, brain, heart brown fat and muscles, and the SUV was calculated. We finally compared choline i.v. standard acquisition to choline RO standard acquisition; choline i.v. delayed acquisition to choline RO delayed acquisition; FDG i.v. standard acquisition to FDG RO standard acquisition; FDG i.v. delayed acquisition to FDG RO delayed acquisition.

RESULTS

The RO injections for both (18)F-FDG and (11)C-choline were comparable to the intravenous injection of F-FDG for the standard and delayed acquisitions.

CONCLUSION

The RO administration in mice represents a technical advantage over intravenous administration in being an easier and faster procedure. However, its use requires high specific activity while its value in peptides and other receptor-specific radiopharmaceuticals needs further assessment.

摘要

背景与目的

小动物正电子发射断层扫描（PET）在临床前研究中越来越重要。在啮齿动物中，放射性示踪剂通常通过尾静脉注射。由于软组织外渗非常频繁且尾部疤痕可能会妨碍初次注射失败后的重复注射，该操作可能非常困难且耗时。我们研究的目的是比较眼眶后（RO）注射与尾静脉静脉内（i.v.）注射（18）F - 氟代脱氧葡萄糖（FDG）和（11）C - 胆碱在小鼠体内用于小动物PET研究的情况。

方法

我们按照以下方案评估了4只健康的雌性ICR CD1小鼠。第1天：每只动物接受28 MBq的（11）C - 胆碱静脉注射。在10分钟和40分钟后进行PET扫描。第2天：每只动物接受28 MBq的（11）C - 胆碱眼眶后注射。在10分钟和40分钟后进行PET扫描。第3天：每只动物接受28 MBq的（18）F - FDG静脉注射。在60分钟和120分钟后进行PET扫描。第4天：每只动物接受28 MBq的（18）F - FDG眼眶后注射。在60分钟和120分钟后进行PET扫描。给药和图像采集在气体麻醉下进行。对于FDG研究，动物禁食2小时，并在注射后保持睡眠20 - 30分钟，以避免肌肉摄取。图像用二维有序子集期望最大化（OSEM）算法重建。对于每次扫描，在肝脏、肾脏、肺、脑、心脏、棕色脂肪和肌肉上绘制感兴趣区（ROI），并计算标准化摄取值（SUV）。我们最终比较了胆碱静脉注射标准采集与胆碱眼眶后标准采集；胆碱静脉注射延迟采集与胆碱眼眶后延迟采集；FDG静脉注射标准采集与FDG眼眶后标准采集；FDG静脉注射延迟采集与FDG眼眶后延迟采集。