Pedras M Soledade C, Chumala Paulos B, Yu Yang
Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK S7N 5C9, Canada.
Can J Microbiol. 2007 Mar;53(3):364-71. doi: 10.1139/W06-133.
Previous molecular chemotaxonomic analyses of isolates of the plant pathogenic fungus Leptosphaeria maculans (Desm.) Ces. et de Not. (asexual stage Phoma lingam (Tode ex Fr.) Desm.) in a chemically defined medium suggested that this species complex was composed of at least three distinct groups. Subsequently, a group within L. maculans was classified as Leptosphaeria biglobosa, on the basis of morphologic characteristics and the lack of sexual crossing. To obtain clarification regarding the metabolite profiles of the various groups or species of blackleg fungi, the objectives of this work were (i) to determine the chemical structures of metabolites produced by Canadian V isolates and Polish-type isolates in potato dextrose broth (PDB) and (ii) to determine the chemotaxonomic relationship among French isolates of L. biglobosa and among Canadian W isolates and Thlaspi isolates of L. maculans. Here, we report for the first time that Canadian V isolates grown in PDB produced 2,4-dihydroxy-3,6-dimethylbenzaldehyde, a metabolite never reported from L. maculans, but none of the usual phytotoxins (sirodesmins). In addition, we report a new metabolite, 2-[2-(5-hydroxybenzofuranyl)]-3-(4-hydroxyphenyl)propanenitrile, from Polish-type isolates of L. maculans grown in PDB and the metabolite profiles of 16 Thlaspi isolates. The metabolite profiles of Thlaspi isolates indicate that these are part of two distinct groups, the Polish W group and the Canadian W group, i.e., L. biglobosa. Finally, we demonstrate that the metabolite profiles of the French isolates classified as L. biglobosa are similar to those of Canadian W isolates.
先前在化学成分明确的培养基中对植物致病真菌大茎点菌（Leptosphaeria maculans (Desm.) Ces. et de Not.，无性阶段为十字花科根肿病菌（Phoma lingam (Tode ex Fr.) Desm.））分离株进行的分子化学分类分析表明，该物种复合体至少由三个不同的组构成。随后，基于形态特征和缺乏有性杂交，大茎点菌中的一个组被归类为双球大茎点菌（Leptosphaeria biglobosa）。为了明确黑胫病菌不同组或物种的代谢物谱，本研究的目的是：（i）确定加拿大V分离株和波兰型分离株在马铃薯葡萄糖肉汤（PDB）中产生的代谢物的化学结构，以及（ii）确定双球大茎点菌法国分离株之间以及加拿大W分离株和大茎点菌遏蓝菜属分离株之间的化学分类关系。在此，我们首次报告，在PDB中培养的加拿大V分离株产生了2,4 - 二羟基 - 3,6 - 二甲基苯甲醛，这是一种从未在大茎点菌中报道过的代谢物，但未产生任何常见的植物毒素（链格孢菌素）。此外，我们报告了一种来自在PDB中培养的大茎点菌波兰型分离株的新代谢物2 - [2 - (5 - 羟基苯并呋喃基)] - 3 - (4 - 羟基苯基)丙腈以及16株遏蓝菜属分离株的代谢物谱。遏蓝菜属分离株的代谢物谱表明，它们分属于两个不同的组，即波兰W组和加拿大W组，也就是双球大茎点菌。最后，我们证明被归类为双球大茎点菌的法国分离株的代谢物谱与加拿大W分离株的相似。
