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侵染冬油菜(甘蓝型油菜)的(某种菌)胞外聚合物(EPS)及其他代谢产物产生的差异 。 (括号内容原文缺失具体信息)

Differences in the Production of Extracellular Polymeric Substances (EPS) and Other Metabolites of () Infecting Winter Oilseed Rape ( L.).

作者信息

Nowak Artur, Kutyła Mateusz, Kaczmarek Joanna, Jaroszuk-Ściseł Jolanta, Jędryczka Małgorzata

机构信息

Department of Industrial and Environmental Microbiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.

Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.

出版信息

Metabolites. 2023 Jun 17;13(6):759. doi: 10.3390/metabo13060759.

Abstract

Species of the genus () are phytopathogens of the Brassicaceae family, which includes oilseed rape. The spores of these fungi spread by airborne transmission, infect plants, and cause crop losses. The secondary metabolism of and was studied and compared, with the main focus being on the ability to produce Extracellular Polymeric Substances (EPS). In spite of the 1.5-2-fold faster growth rate of on Czapek-Dox and other screening media, the average yield of EPS in this fungus was only 0.29 g/L, compared to that of (0.43 g/L). In turn, showed a higher capacity to synthesise IAA, i.e., 14 µg/mL, in contrast to <1.5 µg/mL produced by . On the other hand, the strains showed higher β-glucanase activity (350-400 mU/mL), compared to 50-100 mU/mL in . Invertase levels were similar in both species (250 mU/mL). The positive correlation between invertase activity and EPS yield contrasted with the absence of a correlation of EPS with β-glucanase. neither solubilised phosphate nor used proteins from milk. All strains showed the ability to synthesise siderophores on CAS agar. exhibited the highest efficiency of amylolytic and cellulolytic activity.

摘要

()属的物种是十字花科的植物病原体,十字花科包括油菜。这些真菌的孢子通过空气传播,感染植物并导致作物损失。对和的次生代谢进行了研究和比较,主要关注产生胞外聚合物(EPS)的能力。尽管在查氏培养基和其他筛选培养基上的生长速度比快1.5至2倍,但该真菌中EPS的平均产量仅为0.29克/升,而的平均产量为0.43克/升。反过来,与产生的<1.5微克/毫升相比,显示出更高的合成吲哚-3-乙酸(IAA)的能力,即14微克/毫升。另一方面,菌株显示出更高的β-葡聚糖酶活性(350-400毫单位/毫升),而的β-葡聚糖酶活性为50-100毫单位/毫升。两种菌株的转化酶水平相似(250毫单位/毫升)。转化酶活性与EPS产量之间呈正相关,这与EPS与β-葡聚糖酶之间缺乏相关性形成对比。既不能溶解磷酸盐,也不能利用牛奶中的蛋白质。所有菌株在CAS琼脂上均显示出合成铁载体的能力。表现出最高的淀粉分解和纤维素分解活性效率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d6f/10302272/64e45eef5b53/metabolites-13-00759-g001.jpg

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