Figallo Elisa, Cannizzaro Christopher, Gerecht Sharon, Burdick Jason A, Langer Robert, Elvassore Nicola, Vunjak-Novakovic Gordana
Department of Chemical Engineering, University of Padova, Italy.
Lab Chip. 2007 Jun;7(6):710-9. doi: 10.1039/b700063d. Epub 2007 May 15.
High throughput experiments can be used to spatially and temporally investigate the many factors that regulate cell differentiation. We have developed a micro-bioreactor array (MBA) that is fabricated using soft lithography and contains twelve independent micro-bioreactors perfused with culture medium. The MBA enables cultivation of cells that are either attached to substrates or encapsulated in hydrogels, at variable levels of hydrodynamic shear, and with automated image analysis of the expression of cell differentiation markers. The flow and mass transport in the MBA were characterized by computational fluid dynamic (CFD) modeling. The representative MBA configurations were validated using the C2C12 cell line, primary rat cardiac myocytes and human embryonic stem cells (hESCs) (lines H09 and H13). To illustrate the utility of the MBA for controlled studies of hESCs, we established correlations between the expression of smooth muscle actin and cell density for three different flow configurations.
高通量实验可用于在空间和时间上研究调控细胞分化的诸多因素。我们开发了一种微生物反应器阵列（MBA），它采用软光刻技术制造，包含十二个独立的微生物反应器，由培养基灌注。该MBA能够培养附着于基质或包裹在水凝胶中的细胞，具有可变水平的流体动力剪切力，并能对细胞分化标志物的表达进行自动图像分析。通过计算流体动力学（CFD）建模对MBA中的流动和传质进行了表征。使用C2C12细胞系、原代大鼠心肌细胞和人类胚胎干细胞（hESCs）（H09和H13系）对代表性的MBA配置进行了验证。为了说明MBA在hESCs对照研究中的实用性，我们建立了三种不同流动配置下平滑肌肌动蛋白表达与细胞密度之间的相关性。
