Kudriavtsev V A, Safronova L A, Osadchaia A I, Kozachko I A
Mikrobiol Zh (1978). 1991 Jul-Aug;53(4):68-73.
Nutrient medium chosen as a basic one after preliminary test of several media known from literature has been optimized to intensify biosynthesis and amine nitrogen production by three strains of aerobic sporulating bacteria to culture liquid. The method of mathematical planning used in the experiments has permitted obtaining the components ratio for the medium on which production of amine nitrogen to the environment increased 2.3-3.2 times. The best variants of the optimized medium promoted an increase of the aminosynthetic activity of the studied bacteria by more than 320%. The obtained nutrient medium is appropriate for a wide screening of aerobic bacilli for their ability to synthesize amino compounds.
在对文献中已知的几种培养基进行初步测试后,选择了一种基础培养基,并对其进行了优化,以增强三株需氧芽孢杆菌向培养液中进行生物合成和产生胺态氮的能力。实验中使用的数学规划方法使得能够获得培养基的成分比例,在此比例下,向环境中产生的胺态氮增加了2.3至3.2倍。优化培养基的最佳配方使所研究细菌的氨基合成活性提高了320%以上。所获得的营养培养基适用于广泛筛选需氧芽孢杆菌合成氨基化合物的能力。