通过脉冲场凝胶电泳和显微镜凝集试验鉴定钩端螺旋体属临床分离株
Identification of clinical isolates of Leptospira spp by pulsed field gel-electrophoresis and microscopic agglutination test.
作者信息
Naigowit Pimjai, Charoenchai Sathapana, Biaklang Mayurachat, Seena Umaporn, Wangroongsarb Piyada, Sawanpanyalert Pathom, Warachit Paijit
机构信息
Technical Office, Department of Medical Sciences, Ministry of Public Health, Tiwanon Road, Nonthaburi 11000, Thailand.
出版信息
Southeast Asian J Trop Med Public Health. 2007 Jan;38(1):97-103.
PMID:17539253
Abstract
Twenty-five clinical isolates of Leptospira spp were characterized by microscopic agglutination test (MAT) and pulsed field gel-electrophoresis (PFGE) in comparison with 23 reference Leptospira serovars and with the saprophytic L. biflexa serovar Patoc. PFGE DNA profiling was more specific and reliable than MAT.
摘要
通过显微镜凝集试验（MAT）和脉冲场凝胶电泳（PFGE）对25株钩端螺旋体临床分离株进行了特征分析，并与23株参考钩端螺旋体血清型以及腐生型双曲钩端螺旋体血清型Patoc进行了比较。PFGE DNA图谱分析比MAT更具特异性和可靠性。
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