从印度尼西亚西苏门答腊的菲律宾蚬中分离出的副溶血性弧菌中tdh和trh基因的检测。
Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia.
作者信息
Radu Son, Kqueen Cheah Yoke, Napis Suhaimi, Zakaria Zunita, Mutalib Sahilah Abd, Nishibuchi Mitsuaki
机构信息
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Andalas, Kampus Limau Manis, Padang, Indonesia.
出版信息
Southeast Asian J Trop Med Public Health. 2007 Mar;38(2):349-55.
The occurrence of Vibrio parahaemolyticus in raw Corbicula moltkiana Prime from Lake Singkarak and Pasar Raya Padang market and in cooked samples in West Sumatera, Indonesia, was studied. Thirteen raw and seven cooked bivalve samples were positive using CHROMAgar Vibrio medium. All 47 V parahaemolyticus isolates were positive for toxR gene but negative for trh. However, 36% (17/47) of V parahaemolyticus strains were positive for tdh gene. Antibiotic profiling showed that 76% and 38% of isolates from raw and cooked bivalves respectively were resistant to ampicillin. Using RAPD-PCR analysis, most of the strains were clustered according to their source of isolation but some of the strains from raw and cooked samples were clustered together. These results indicate that pathogenic V parahaemolyticus isolates are present in Corbicula moltkiana Prime in West Sumatera, Indonesia, suggesting that V parahaemolyticus may also be present in seafood in other regions of Indonesia.
对印度尼西亚西苏门答腊省辛卡拉湖和巴东中央市场的新鲜无齿蚌以及熟食样本中副溶血性弧菌的存在情况进行了研究。使用CHROMAgar弧菌培养基检测，13份生鲜双壳贝类样本和7份熟食样本呈阳性。所有47株副溶血性弧菌分离株toxR基因呈阳性，但trh基因呈阴性。然而，36%（17/47）的副溶血性弧菌菌株tdh基因呈阳性。抗生素谱分析表明，分别从生鲜和熟食双壳贝类分离出的菌株中，76%和38%对氨苄青霉素耐药。使用随机扩增多态性DNA聚合酶链反应（RAPD-PCR）分析，大多数菌株根据其分离来源聚类，但一些生鲜和熟食样本中的菌株聚集在一起。这些结果表明，印度尼西亚西苏门答腊省的无齿蚌中存在致病性副溶血性弧菌分离株，这表明印度尼西亚其他地区的海鲜中可能也存在副溶血性弧菌。
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