Department of Microbiology, All India Institute of Hygiene and Public Health, Kolkata, India.
Eur Rev Med Pharmacol Sci. 2010 Jun;14(6):545-9.
Vibrio parahaemolyticus is a marine bacterium which is also responsible for acute diarrhoeal illness in human beings. Eating raw seafish or contaminated seafood is responsible for acute gastroenteritis. The aim of this study was to investigate the isolation, identification and molecular characterization of Vibrio parahaemolyticus from the fish samples in Kolkata, India.
In this study 90 fish samples were collected from 8 different market places in Kolkata, India. Fish samples collected were shrimp, prawn, bhetki, pamfret and hilsa. VP-toxR PCR was performed to confirm the presence of species specific toxR. tdh and trh genes PCR for detection of virulence genes were performed separately. GS-PCR was performed in tdh, trh gene positive strains to determine whether they belong to pandemic genotype. Serotyping was also done on the tdh, trh positive strains.
Out of the 90 fish samples collected from different local fish markets 60 were positive for Vibrio parahaemolyticus. 21 (35%) out of 60 Vibrio parahaemolyticus isolates from fish samples harboured the tdh gene. 1 (1.7%) out of 60 Vibrio parahaemolyticus isolates from fish samples carried trh gene. Out of 22 isolates only 2 were positive for GS-PCR. O10:KUT was the serovar maximum isolated.
Considerable percentage of Vibrio parahaemolyticus carrying the virulence genes and pandemic genotype among fish in Kolkata indicates that there is potential reservoir in Kolkata and consumption of sea fish or contaminated fish might cause Vibrio parahaemolyticus mediated diarrhea in this region.
副溶血性弧菌是一种海洋细菌，也可导致人类急性腹泻病。食用生鱼片或受污染的海鲜可导致急性肠胃炎。本研究旨在调查印度加尔各答鱼类样本中副溶血性弧菌的分离、鉴定和分子特征。
本研究共采集了印度加尔各答 8 个不同市场的 90 个鱼类样本。采集的鱼类样本包括虾、对虾、比目鱼、石斑鱼和鲱鱼。VP-toxR PCR 用于确认物种特异性 toxR 的存在。分别进行 tdh 和 trh 基因 PCR 检测毒力基因。在 tdh、trh 基因阳性菌株中进行 GS-PCR，以确定它们是否属于流行基因型。对 tdh、trh 阳性菌株进行血清型分型。
从不同当地鱼市场采集的 90 个鱼类样本中，有 60 个样本检测出副溶血性弧菌阳性。从鱼类样本中分离的 60 株副溶血性弧菌中有 21 株（35%）携带 tdh 基因。从鱼类样本中分离的 60 株副溶血性弧菌中有 1 株（1.7%）携带 trh 基因。在 22 个分离株中，只有 2 个对 GS-PCR 呈阳性。O10:KUT 是分离株最多的血清型。
加尔各答鱼类携带毒力基因和流行基因型的副溶血性弧菌比例相当高，表明加尔各答存在潜在的储存库，食用海鱼或受污染的鱼类可能导致该地区副溶血性弧菌介导的腹泻。
