[Study on HPEC fingerprint of cultured Cordyceps militaris].

OBJECTIVE

To investigate the High Performance Capillary Electrophoresis (HPCE) fingerprint of cultured Cordyceps militaris (L.) Link.

METHODS

Separation was performed on a 50 cm x 75 microm uncoated capillary with 0.5 mmol/L borate solution (pH 9. 18) as HPEC buffer. The run voltage was 20 Kv, temperature 25 degrees C and the DAD detection was set at 254nm.

RESULTS

Fingerprint consisted of 11 common peaks. The validation of methods was satisfied with the requirements for SFDA's technical regulations.

CONCLUSION

The method was accurate and simple and suitable to the quality control of cultured Cordyceps militaris (L.) Link.