Li Hong, Du Qun, Wang Wen-jun, Wang Ru-jun, Wang Jian-hua, Liu Shan-shan
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Zhong Yao Cai. 2007 Jan;30(1):56-9.
To investigate the effect of Kuijieling Decoction (KD) on gene expression of Toll-like receptor (TLR) 2,4 in colonic mucosa of ulcerative colitis( UC) rats.
48 rats were divided into six groups as follows: normal control (NC) group, model control (MC) group, Kuijieling low dose (KLD) group, Kuijieling medium dose (KMD) group, Kuijieling high dose (KHD) group and SASP group. TLR2/4 gene expression in colonic mucosa was measured by reverse-transcription polymerase chain reaction (RT-PCR).
Relative gene expression of TLR2/4 in MC group were significantly higher than that in NC group (P < 0.01). Relative gene expression of TLR2 in KMD and KHD group was significantly lower than that in MC group (P < 0.05). Relative gene expression of TLR4 in KHD group was significantly lower than that in MC group (P < 0.05).
Up-regulation of TLR2 and TLR4 gene expression can be seen in colonic mucosa of UC rats. KD can inhibit the gene expression of TLR2 and TLR4. The inhibitory effects of KD on UC may be associated with the inhibition of TLR2 and TLR4 gene expression.
探讨溃结灵汤对溃疡性结肠炎（UC）大鼠结肠黏膜Toll样受体（TLR）2、4基因表达的影响。
将48只大鼠分为6组：正常对照组（NC组）、模型对照组（MC组）、溃结灵低剂量组（KLD组）、溃结灵中剂量组（KMD组）、溃结灵高剂量组（KHD组）和柳氮磺胺吡啶组。采用逆转录聚合酶链反应（RT-PCR）检测结肠黏膜中TLR2/4基因表达。
MC组TLR2/4相对基因表达显著高于NC组（P<0.01）。KMD组和KHD组TLR2相对基因表达显著低于MC组（P<0.05）。KHD组TLR4相对基因表达显著低于MC组（P<0.05）。
UC大鼠结肠黏膜可见TLR2和TLR4基因表达上调。溃结灵汤可抑制TLR2和TLR4基因表达。溃结灵汤对UC的抑制作用可能与抑制TLR2和TLR4基因表达有关。
