溃结灵汤对溃疡性结肠炎模型大鼠结肠黏膜IKK-α蛋白表达的影响
[Effect of Kuijieling decoction on proteins expression of IKK-alpha in colonic mucosa of ulcerative colitis model rats].
作者信息
Du Qun, Li Hong, Wang Ru-Jun, Li Yan-Wu, Wu Yan-Li, Wang Jian-Hua
机构信息
Pi-wei institute, Guangzhou University of TCM, Guangzhou 510405, China.
出版信息
Zhong Yao Cai. 2008 Jul;31(7):1016-8.
OBJECTIVE
To explore the changes of protein expression of IKK-alpha as well as the effects of Kuijieling Decoction (KD) on colonic mucosa of ulcerative colitis (UC) model rats.
METHODS
UC model rats were induced by TNBS. The rats were randomly divided into six groups: normal control (NC) group, model control (MC) group, Kuijieling low dose (KLD), middle dose (KMD) group, high dose (KHD) group and SASP group. After 10-days' treatment the rats were killed to get their colonic tissues. The positive rate of IKK-alpha expression was detected by immunohistochemical (IHC).
RESULTS
The positive rate of IKK-alpha in MC group was significantly higher than that in NC group (P < 0.01). The positive rate of IKK-alpha in KMD group was significantly lower than that in MC group (P < 0.05). The positive rate of IKK-alpha in KHD and SASP group were significantly lower than that in MC group (P < 0.01).
CONCLUSION
IKK-alpha may be involved in the pathogenesis of UC, and KD can inhibit positive rate of IKK-alpha in colonic mucosa of UC model rats induced by TNBS. The inhibitory effects of KD on UC may be associated with this.
目的
探讨IKK-α蛋白表达变化以及溃结灵（KD）对溃疡性结肠炎（UC）模型大鼠结肠黏膜的影响。
方法
采用三硝基苯磺酸（TNBS）诱导UC模型大鼠。将大鼠随机分为六组：正常对照组（NC）、模型对照组（MC）、溃结灵低剂量组（KLD）、中剂量组（KMD）、高剂量组（KHD）和柳氮磺胺吡啶（SASP）组。治疗10天后处死大鼠，获取结肠组织。采用免疫组织化学（IHC）检测IKK-α表达阳性率。
结果
MC组IKK-α阳性率显著高于NC组（P < 0.01）。KMD组IKK-α阳性率显著低于MC组（P < 0.05）。KHD组和SASP组IKK-α阳性率显著低于MC组（P < 0.01）。
结论
IKK-α可能参与UC的发病机制，KD可抑制TNBS诱导的UC模型大鼠结肠黏膜IKK-α阳性率。KD对UC的抑制作用可能与此有关。
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