Tsuyama Shoichiro, Oikawa Daichi, Yamasaki Yasuko, Takagi Sayuri, Ando Hironori, Furuse Mitsuhiro
Laboratory of Advanced Animal and Marine Bioresources, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.
Nutr Neurosci. 2007 Feb-Apr;10(1-2):45-50. doi: 10.1080/10284150701250671.
The two principal endocannabinoids, N-arachidonoyl-ethanolamine (anandamide) and 2-arachidonoyl-glycerol (2-AG), are synthesized from arachidonic acid (AA) and AA is released as they are degraded. Therefore, the function of endocannabinoids is closely linked to AA, but the exact relationships have not been clarified, especially with respect to endocannabinoid metabolism. In the present study, oil rich in AA was administered (0, 100, 200 and 300 microl) orally to male mice for 7 days. Phospholipase D (PLD), fatty acid amide hydrolase (FAAH), diacyl-glycerol lipase (DAGL), monoacyl-glycerol lipase (MAGL) and cannabinoid 1 (CB1) receptor mRNA expressions were determined in the whole brain. No changes in the expression of any gene investigated were detected following AA treatment. However, it was demonstrated that the expression of the CB1 receptor was positively correlated with PLD, FAAH and DAGL expression. This suggests that expression of the CB1 receptor is closely coordinated with that of the enzymes which synthesize its ligands.
两种主要的内源性大麻素，N-花生四烯酰乙醇胺（花生四烯酸乙醇胺）和2-花生四烯酸甘油酯（2-AG），由花生四烯酸（AA）合成，且在它们降解时AA会被释放出来。因此，内源性大麻素的功能与AA密切相关，但确切关系尚未阐明，尤其是在内源性大麻素代谢方面。在本研究中，给雄性小鼠口服富含AA的油（0、100、200和300微升），持续7天。测定全脑中磷脂酶D（PLD）、脂肪酸酰胺水解酶（FAAH）、二酰基甘油脂肪酶（DAGL）、单酰基甘油脂肪酶（MAGL）和大麻素1（CB1）受体mRNA的表达。AA处理后，未检测到所研究的任何基因表达有变化。然而，已证明CB1受体的表达与PLD、FAAH和DAGL的表达呈正相关。这表明CB1受体的表达与其配体合成酶的表达密切协调。
