Aleshkov S B, Ustav M B, Baev A A
Mol Biol (Mosk). 1991 Sep-Oct;25(5):1226-38.
A number of recombinant cell lines which produce human tissue plasminogen activator (tPA) was obtained using different hosts--mouse, rat, hamster, simian and human cell lines. All types of recombinant lines secreted active r-tPA into conditioned medium. A slight difference between molecular weights of secreted variants of r-tPA was mediated by the different mechanisms of protein modification. Treatment of some recombinant cell lines with different substances resulted in increased levels of r-tPA production.
使用不同宿主——小鼠、大鼠、仓鼠、猴和人细胞系，获得了多个产生人组织型纤溶酶原激活剂（tPA）的重组细胞系。所有类型的重组细胞系均将活性重组tPA分泌到条件培养基中。r-tPA分泌变体分子量的细微差异是由蛋白质修饰的不同机制介导的。用不同物质处理一些重组细胞系会导致r-tPA产量增加。
