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肿瘤促进剂佛波酯(TPA)对哺乳动物细胞培养系统中纤溶酶原激活物生成、磷脂酰胆碱合成及己糖转运的影响之间缺乏相关性。

Lack of correlation between effects of tumor promoter TPA on plasminogen activator production, phosphatidyl choline synthesis, and hexose transport in mammalian cell culture systems.

作者信息

Plagemann P G, Estensen R D

出版信息

J Cell Physiol. 1980 Jul;104(1):105-19. doi: 10.1002/jcp.1041040114.

Abstract

We have investigated the effects of the tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate (TPA) on plasminogen activator production, hexose transport and metabolism, and the incorporation of choline into the acid soluble pool and into phosphatidylcholine in suspension cultures of mouse L, mouse P388 leukemia, human HeLa, and Chinese hamster ovary cells, and in monolayer cultures of baby hamster kidney (BHK), mouse 3T3, mouse 3T6, and mouse P388D1 macrophage-like cells. BHK, 3T3, P388D1, and P388 cells produced plasminogen activator constitutively, but no significant production was observed in the other cell lines. Plasminogen activator production was induced or stimulated by TPA only in P388 cells (10- to 20-fold by 100 ng TPA/ml). On the other hand, phosphatidylcholine synthesis was stimulated by TPA only in HeLa cells, and hexose transport, as measured with 3-0-methyl-D-glucose, only in 3T3 and P388D1 cells, as well as in human lymphocytes. The stimulation of hexose transport occurred more rapidly than the induction of plasminogen activator production and seemed to be part of the mitogenic response of cells to TPA treatment. A stimulation of deoxyglucose uptake was similarly limited to 3T3 and P388D1 cells. A significant decarboxylation of carbon 1 of deoxyglucose occurred in P388 and P388D1 cells, but not in Novikoff cells, and any decarboxylation that occurred was not stimulated by TPA. The results indicate that the various investigated responses of cells to TPA are unrelated and occur independent of each other. The time courses of the biochemical responses also differ significantly.

摘要

我们研究了肿瘤促进剂12 - 0 - 十四烷酰佛波醇-13 - 乙酸酯(TPA)对小鼠L细胞、小鼠P388白血病细胞、人HeLa细胞和中国仓鼠卵巢细胞悬浮培养物,以及幼仓鼠肾(BHK)细胞、小鼠3T3细胞、小鼠3T6细胞和小鼠P388D1巨噬细胞样细胞单层培养物中纤溶酶原激活物产生、己糖转运与代谢,以及胆碱掺入酸溶性池和磷脂酰胆碱的影响。BHK、3T3、P388D1和P388细胞组成性产生纤溶酶原激活物,但在其他细胞系中未观察到显著产生。仅在P388细胞中,TPA诱导或刺激了纤溶酶原激活物的产生(100 ng TPA/ml可使其增加10至20倍)。另一方面,仅在HeLa细胞中,TPA刺激了磷脂酰胆碱的合成;仅在3T3和P388D1细胞以及人淋巴细胞中,用3 - O - 甲基 - D - 葡萄糖测量时,TPA刺激了己糖转运。己糖转运的刺激比纤溶酶原激活物产生的诱导更快发生,并且似乎是细胞对TPA处理的促有丝分裂反应的一部分。脱氧葡萄糖摄取的刺激同样仅限于3T3和P388D1细胞。在P388和P388D1细胞中发生了脱氧葡萄糖1 - 碳的显著脱羧,但在诺维科夫细胞中未发生,并且发生的任何脱羧都未被TPA刺激。结果表明,细胞对TPA的各种研究反应是不相关的,并且彼此独立发生。生化反应的时间进程也有显著差异。

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