Department of Endocrinology, Shaanxi Provincial People's Hospital.
Cardiol J. 2020;27(6):807-816. doi: 10.5603/CJ.a2018.0113. Epub 2018 Sep 24.
The study aimed to uncover the regulation mechanisms of diabetic cardiomyopathy (DCM) and provide novel prognostic biomarkers.
The dataset GSE62203 downloaded from the Gene Expression Omnibus database was utilized in the present study. After pretreatment using the Affy package, differentially expressed genes (DEGs) were identified by the limma package, followed by functional enrichment analysis and protein- protein interaction (PPI) network analysis. Furthermore, module analysis was conducted using MCODE plug-in of Cytoscape, and functional enrichment analysis was also performed for genes in the modules.
A set of 560 DEGs were screened, mainly enriched in the metabolic process and cell cycle related process. Hub nodes in the PPI network were LDHA (lactate dehydrogenase A), ALDOC (aldolase C, fructose-bisphosphate) and ABCE1 (ATP Binding Cassette Subfamily E Member 1), which were also highlighted in Module 1 or Module 2 and predominantly enriched in the processes of glycolysis and ribosome biogenesis. Additionally, LDHA were linked with ALDOC in the PPI network. Besides, activating transcription factor 4 (ATF4) was prominent in Module 3; while myosin heavy chain 6 (MYH6) was highlighted in Module 4 and was mainly involved in muscle cells related biological processes.
Five potential biomarkers including LDHA, ALDOC, ABCE1, ATF4 and MYH6 were identified for DCM prognosis.
本研究旨在揭示糖尿病心肌病（DCM）的调控机制，并提供新的预后生物标志物。
本研究使用从基因表达综合数据库（Gene Expression Omnibus database）下载的数据集 GSE62203。使用 Affy 包进行预处理后，通过 limma 包鉴定差异表达基因（DEGs），然后进行功能富集分析和蛋白质-蛋白质相互作用（PPI）网络分析。此外，使用 Cytoscape 的 MCODE 插件进行模块分析，并对模块中的基因进行功能富集分析。
筛选出一组 560 个 DEGs，主要富集在代谢过程和细胞周期相关过程中。PPI 网络中的枢纽节点为 LDHA（乳酸脱氢酶 A）、ALDOC（醛缩酶 C，果糖-二磷酸）和 ABCE1（ATP 结合盒亚家族 E 成员 1），它们也在模块 1 或模块 2 中突出显示，主要富集在糖酵解和核糖体生物发生过程中。此外，LDHA 在 PPI 网络中与 ALDOC 相关。此外，激活转录因子 4（ATF4）在模块 3 中突出；而肌球蛋白重链 6（MYH6）在模块 4 中突出，主要参与肌肉细胞相关的生物学过程。
本研究确定了 5 个潜在的生物标志物，包括 LDHA、ALDOC、ABCE1、ATF4 和 MYH6，可用于 DCM 的预后。
