Ivanova Pavlina T, Milne Stephen B, Forrester Jeffrey S, Brown H Alex
Department of Pharmacology and the Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Mol Interv. 2004 Apr;4(2):86-96. doi: 10.1124/mi.4.2.6.
Phospholipids are the structural building blocks of the membrane bilayer, which retains and regulates intra-cellular content. In addition to creating a protective barrier around the cell, lipids modulate membrane trafficking and are themselves precursors of important intracellular signaling molecules. Identification and quantification of these molecular species is essential for a more complete understanding of cell signaling pathways, and more reliable and sensitive methods are needed for determining membrane phospholipid content. Recent improvements in electrospray ionization mass spectrometry have made possible the direct identification of more than 400 phospholipid species from biological extracts of a single cell type. Changes in the cellular concentration of diverse lipids can be determined by analysis of the mass spectra by statistical algorithms. In the future, lipid arrays will be integrated with other high-throughput profiling technologies, and computational lipidomics will expand our understanding of the molecular basis of cellular processes and diseases.
磷脂是膜双层结构的组成基石，可保留并调节细胞内物质。除了在细胞周围形成保护屏障外，脂质还调节膜运输，并且自身也是重要细胞内信号分子的前体。识别和定量这些分子种类对于更全面地理解细胞信号通路至关重要，因此需要更可靠、更灵敏的方法来测定膜磷脂含量。电喷雾电离质谱技术的最新进展使得从单一细胞类型的生物提取物中直接鉴定出400多种磷脂种类成为可能。通过统计算法分析质谱图可以确定不同脂质的细胞浓度变化。未来，脂质阵列将与其他高通量分析技术相结合，计算脂质组学将拓展我们对细胞过程和疾病分子基础的理解。
