Yoshii Hironori, Somboonthum Pranee, Takahashi Michiaki, Yamanishi Koichi, Mori Yasuko
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Vaccine. 2007 Jun 28;25(27):5006-12. doi: 10.1016/j.vaccine.2007.04.064. Epub 2007 May 7.
The complete genome of the varicella-zoster virus (VZV) Oka vaccine strain (vOka) has been cloned into a bacterial artificial chromosome (BAC), and several BAC clones with the vOka genome have been obtained. Infectious viruses were reconstituted in MRC-5 cells transfected with the vOka-BAC DNA clones. The clones were distributed into two groups based on differences in amino acids found in ORF 62/71 region among the vOka-BAC clones. The recombinant vOka (rvOka) grew slower than recombinant Oka parental virus (rpOka), pOka and vOka. This is the first report that the vOka genome was cloned into BAC vector. The rvOka-BAC system would be useful as a vector for construction of recombinant live vaccines.
水痘带状疱疹病毒（VZV）Oka疫苗株（vOka）的完整基因组已被克隆到细菌人工染色体（BAC）中，并获得了几个含有vOka基因组的BAC克隆。用vOka-BAC DNA克隆转染MRC-5细胞后，重组出了感染性病毒。根据vOka-BAC克隆中ORF 62/71区域发现的氨基酸差异，将这些克隆分为两组。重组vOka（rvOka）的生长速度比重组Oka亲本病毒（rpOka）、pOka和vOka慢。这是首次将vOka基因组克隆到BAC载体中的报道。rvOka-BAC系统作为构建重组活疫苗的载体将很有用。
