Beekes Michael, Lasch Peter, Naumann Dieter
P24 - Transmissible Spongiform Encephalopathies, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany.
Vet Microbiol. 2007 Aug 31;123(4):305-19. doi: 10.1016/j.vetmic.2007.04.010. Epub 2007 Apr 8.
A genuine biophysical method, Fourier transform-infrared (FT-IR) spectroscopy has become a versatile research tool in biochemistry and biomedicine. Topical applications in microbiology and prion research are impressive illustrations of the vigorous evolution of the technique. FT-IR spectroscopy has established itself as a powerful method for the rapid differentiation and identification of microorganisms, thereby contributing to both clinical medicine and the prevention of bioterrorism. It has also led to considerable progress in various other fields of basic research, not least in prion sciences. In this field, FT-IR spectroscopy has been increasingly applied as a tool for elucidating structural features of the pathological prion protein, and also to study the molecular changes induced by prions in neuronal tissue and blood. This article sets out to give a review of current examples of the analytical potential of FT-IR spectroscopy in microbiology and prion research.
傅里叶变换红外（FT-IR）光谱法作为一种真正的生物物理方法，已成为生物化学和生物医学领域中一种用途广泛的研究工具。在微生物学和朊病毒研究中的局部应用，是该技术蓬勃发展的生动例证。FT-IR光谱法已成为快速鉴别和识别微生物的有力方法，从而为临床医学和生物恐怖主义防范做出了贡献。它还在基础研究的各个其他领域取得了显著进展，尤其是在朊病毒科学领域。在该领域，FT-IR光谱法越来越多地被用作阐明病理性朊病毒蛋白结构特征的工具，也用于研究朊病毒在神经元组织和血液中引起的分子变化。本文旨在综述FT-IR光谱法在微生物学和朊病毒研究中的分析潜力的当前实例。
