一种用于测定局部用纳米乳剂中槲皮素和甲基槲皮素的等度液相色谱法的验证

Validation of an isocratic LC method for determination of quercetin and methylquercetin in topical nanoemulsions.

作者信息

Fasolo Daniel, Schwingel Liege, Holzschuh Maribete, Bassani Valquíria, Teixeira Helder

机构信息

Universidade Federal do Rio Grande do Sul, Faculdade de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Porto Alegre, RS, Brazil.

出版信息

J Pharm Biomed Anal. 2007 Sep 3;44(5):1174-7. doi: 10.1016/j.jpba.2007.04.026. Epub 2007 Apr 24.

DOI:10.1016/j.jpba.2007.04.026

PMID:17540529

Abstract

The aim of this study was to validate an isocratic LC method for the quantification of either quercetin (Q) or methylquercetin (MQ) incorporated in topical nanoemulsions. The analyses were performed at room temperature on a reversed-phase C(18) column using a mobile phase composed of methanol/water (70:30, v/v) and trifluoracetic acid 0.1% at 0.8 mL min(-1). The detection was carried out on a UV detector at 368 or 354 nm for Q and MQ, respectively. The linearity, in the range of 0.15-1.5 microg/mL, presented a determination coefficient (r(2)) higher than 0.99, calculated by the least square method for both flavonoids. No interferences from the excipients (egg-lecithin or octyldodecanol) were detected. The R.S.D. values for intra- and inter-day precision experiments were lower than 2% for both flavonoids. The recovery ranged from 98.9% to 103.46% for Q and from 98.9% to 102.92% for MQ.

摘要

本研究的目的是验证一种等度液相色谱法，用于定量测定局部用纳米乳剂中所含的槲皮素（Q）或甲基槲皮素（MQ）。分析在室温下于反相C(18)柱上进行，使用由甲醇/水（70:30，v/v）和0.1%三氟乙酸组成的流动相，流速为0.8 mL min(-1)。分别在368 nm或354 nm波长下用紫外检测器对Q和MQ进行检测。在0.15 - 1.5 μg/mL范围内，通过最小二乘法计算，两种黄酮类化合物的线性关系的决定系数（r(2)）均高于0.99。未检测到辅料（卵磷脂或辛基十二醇）的干扰。两种黄酮类化合物日内和日间精密度实验的相对标准偏差（R.S.D.）值均低于2%。Q的回收率在98.9%至103.46%之间，MQ的回收率在98.9%至102.92%之间。