米诺环素与黏菌素联合使用对耐亚胺培南鲍曼不动杆菌临床分离株的体外作用
In vitro effect of minocycline and colistin combinations on imipenem-resistant Acinetobacter baumannii clinical isolates.
作者信息
Tan Thean Yen, Ng Lily Siew Yong, Tan Eugene, Huang Gary
机构信息
Laboratory Medicine Services, Changi General Hospital, 2 Simei Street 3, Singapore 529889.
出版信息
J Antimicrob Chemother. 2007 Aug;60(2):421-3. doi: 10.1093/jac/dkm178. Epub 2007 May 31.
OBJECTIVES
The study investigated the effect of colistin and minocycline when tested singly and in combination against Acinetobacter baumannii.
METHODS
Thirteen unrelated imipenem-resistant A. baumannii clinical isolates were included in the study. MICs of colistin sulphate and minocycline were determined by broth macrodilution and Etest. Organisms were also tested against the two antibiotics singly and in combination using time-kill methods and an Etest-based method.
RESULTS
Neither colistin nor minocycline when tested alone demonstrated bactericidal activity. However, the combination of colistin and minocycline demonstrated bactericidal activity against most of the isolates tested. At 24 h, the combination of antibiotics demonstrated synergy in 12 of the 13 isolates by time-kill methods. None of the isolates demonstrated synergy by Etest methods.
CONCLUSIONS
The combination of colistin and minocycline was found to be bactericidal and synergistic against A. baumannii by time-kill methods. There was no agreement between time-kill and Etest methods for synergy testing.
目的
本研究调查了单独使用及联合使用黏菌素和米诺环素对鲍曼不动杆菌的作用。
方法
本研究纳入了13株无亲缘关系的耐亚胺培南鲍曼不动杆菌临床分离株。采用肉汤稀释法和Etest法测定硫酸黏菌素和米诺环素的最低抑菌浓度（MIC）。还使用时间杀菌法和基于Etest的方法对这些菌株单独及联合使用这两种抗生素进行了检测。
结果
单独检测时，黏菌素和米诺环素均未表现出杀菌活性。然而，黏菌素和米诺环素联合使用时，对大多数检测的分离株表现出杀菌活性。在24小时时，通过时间杀菌法，13株分离株中有12株显示联合使用抗生素具有协同作用。通过Etest法检测，没有分离株显示协同作用。
结论
通过时间杀菌法发现，黏菌素和米诺环素联合使用对鲍曼不动杆菌具有杀菌作用且具有协同作用。在协同作用检测方面，时间杀菌法和Etest法的结果不一致。
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