Ishii I, Mizuta H, Sei A, Hirose J, Kudo S, Hiraki Y
Department of Orthopaedic and Neuromusculoskeletal Surgery, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
J Bone Joint Surg Br. 2007 May;89(5):693-700. doi: 10.1302/0301-620X.89B5.18450.
We have investigated in vitro the release kinetics and bioactivity of fibroblast growth factor-2 (FGF-2) released from a carrier of fibrin sealant. In order to evaluate the effects of the FGF-2 delivery mechanism on the repair of articular cartilage, full-thickness cylindrical defects, 5 mm in diameter and 4 mm in depth, which were too large to undergo spontaneous repair, were created in the femoral trochlea of rabbit knees. These defects were then filled with the sealant. Approximately 50% of the FGF-2 was released from the sealant within 24 hours while its original bioactivity was maintained. The implantation of the fibrin sealant incorporating FGF-2 successfully induced healing of the surface with hyaline cartilage and concomitant repair of the subchondral bone at eight weeks after the creation of the defect. Our findings suggest that this delivery method for FGF-2 may be useful for promoting regenerative repair of full-thickness defects of articular cartilage in humans.
我们已经在体外研究了从纤维蛋白密封剂载体释放的成纤维细胞生长因子-2（FGF-2）的释放动力学和生物活性。为了评估FGF-2递送机制对关节软骨修复的影响，在兔膝关节股骨滑车处制造了直径5毫米、深度4毫米的全层圆柱形缺损，这些缺损太大以至于无法自发修复。然后用密封剂填充这些缺损。约50%的FGF-2在24小时内从密封剂中释放出来，同时保持其原始生物活性。在缺损形成后八周，植入含有FGF-2的纤维蛋白密封剂成功诱导了透明软骨表面的愈合以及软骨下骨的伴随修复。我们的研究结果表明，这种FGF-2递送方法可能有助于促进人类关节软骨全层缺损的再生修复。
