Belayev Ludmila, Obenaus Andre, Zhao Weizhao, Saul Isabel, Busto Raul, Wu Chunyan, Vigdorchik Alexey, Lin Baowan, Ginsberg Myron D
Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami School of Medicine, Miami, FL, USA.
Brain Res. 2007 Jul 9;1157:146-55. doi: 10.1016/j.brainres.2007.04.077. Epub 2007 May 8.
We characterized acute intracerebral hemorrhage (ICH) in the rat by sequential magnetic resonance imaging (MRI) and correlated MRI findings with neurobehavior and histopathology. In addition, we investigated whether albumin treatment would reduce ICH-induced brain injury. ICH was produced in rats by a double-injection method in which 45 microl of fresh arterial blood was injected into the right striatum. Susceptibility-weighted (SWI) and T2-weighted (T2WI) MRI was carried out on a 4.7T magnet at 0-1 h, 6 h, 24 h, 72 h, and 7 days after ICH. Animals were treated with either 25% human albumin, 1.25 g/kg, or saline vehicle i.v. at 90 min after ICH. Neurological status was evaluated before ICH and after treatment (at 4 h, 24 h, 48 h, 72 h, and 7 days). Brains were then perfusion-fixed, re-imaged on an 11.7T magnet, and studied by histopathology and immunochemistry. MRI revealed a consistent hematoma involving the striatum and overlying corpus callosum, with significant volume changes over time. Lesion volumes computed from T2WI images and by histopathology agreed closely with one another and were highly correlated (p=0.002). SWI lesion volumes were also highly correlated to histological volumes (p<0.001) but overestimated histological hematoma volume by approximately 5-fold. Albumin treatment significantly improved neurological scores compared to saline at 72 h (3.8+/-0.6 vs. 1.5+/-0.7) and 7 days (3.8+/-0.4 vs. 1.3+/-0.5, respectively, p<0.05), but did not affect histological or MRI lesion volumes. Taken together, sequential MRI plus histopathology provides a comprehensive characterization of experimental ICH. Albumin treatment improves neurological deficit after ICH but does not affect MRI or histological hematoma size.
我们通过连续磁共振成像（MRI）对大鼠急性脑出血（ICH）进行了特征描述，并将MRI结果与神经行为和组织病理学进行了关联。此外，我们研究了白蛋白治疗是否会减轻ICH诱导的脑损伤。通过双注射法在大鼠中制造ICH，即将45微升新鲜动脉血注入右侧纹状体。在ICH后的0 - 1小时、6小时、24小时、72小时和7天，在4.7T磁体上进行了敏感性加权（SWI）和T2加权（T2WI）MRI检查。在ICH后90分钟，动物接受25%人白蛋白（1.25 g/kg）或生理盐水静脉注射治疗。在ICH前和治疗后（4小时、24小时、48小时、72小时和7天）评估神经状态。然后对大脑进行灌注固定，在11.7T磁体上重新成像，并通过组织病理学和免疫化学进行研究。MRI显示纹状体和上方胼胝体出现一致性血肿，其体积随时间有显著变化。从T2WI图像计算的病变体积与组织病理学结果密切一致且高度相关（p = 0.002）。SWI病变体积也与组织学体积高度相关（p < 0.001），但将组织学血肿体积高估了约5倍。与生理盐水相比，白蛋白治疗在72小时（分别为3.8±0.6对1.5±0.7）和7天（分别为3.8±0.4对1.3±0.5，p < 0.05）时显著改善了神经评分，但不影响组织学或MRI病变体积。综上所述，连续MRI加上组织病理学提供了实验性ICH的全面特征描述。白蛋白治疗可改善ICH后的神经功能缺损，但不影响MRI或组织学血肿大小。
