Department of Medical Physics and Engineering, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita 560-0871, Osaka, Japan.
Department of Advanced Medical Technologies, National Cardiovascular and Cerebral Research Center, Suita 564-8565, Osaka, Japan.
Int J Mol Sci. 2023 Apr 1;24(7):6627. doi: 10.3390/ijms24076627.
This study aimed to evaluate an intracerebral hemorrhage (ICH) model using quantitative susceptibility mapping (QSM) and chemical exchange saturation transfer (CEST) with preclinical 7T-magnetic resonance imaging (MRI) and determine the potential of amide proton transfer-CEST (APT-CEST) for use as a biomarker for the early detection of ICH. Six Wistar male rats underwent MRI, and another six underwent histopathological examinations on postoperative days 0, 3, and 7. The ICH model was created by injecting bacterial collagenase into the right hemisphere of the brain. QSM and APT-CEST MRI were performed using horizontal 7T-MRI. Histological studies were performed to observe ICH and detect iron deposition at the ICH site. T-weighted images (TWI) revealed signal changes associated with hemoglobin degeneration in red blood cells, indicating acute-phase hemorrhage on day 0, late-subacute-phase hemorrhage on day 3, and chronic-phase hemorrhage on day 7. The susceptibility alterations in each phase were detected using QSM. QSM and Berlin blue staining revealed hemosiderin deposition in the chronic phase. APT-CEST revealed high magnetization transfer ratios in the acute phase. Abundant mobile proteins and peptides were observed in early ICH, which were subsequently diluted. APT-CEST imaging may be a reliable noninvasive biomarker for the early diagnosis of ICH.
本研究旨在使用临床前 7T 磁共振成像（MRI）评估脑出血（ICH）模型的定量磁化率映射（QSM）和化学交换饱和转移（CEST），并确定酰胺质子转移-CEST（APT-CEST）作为ICH 早期检测的生物标志物的潜力。六只雄性 Wistar 大鼠接受 MRI 检查，另外六只大鼠在术后第 0、3 和 7 天接受组织病理学检查。ICH 模型通过将细菌胶原酶注入大脑右半球创建。QSM 和 APT-CEST MRI 使用水平 7T-MRI 进行。进行组织学研究以观察 ICH 和检测 ICH 部位的铁沉积。T 加权图像（TWI）显示与红细胞中血红蛋白变性相关的信号变化，表明第 0 天出现急性期出血，第 3 天出现晚期亚急性期出血，第 7 天出现慢性期出血。使用 QSM 检测每个阶段的磁化率变化。QSM 和柏林蓝染色显示慢性期有含铁血黄素沉积。APT-CEST 在急性期显示出较高的磁化转移比。在早期 ICH 中观察到大量的可移动蛋白和肽，随后被稀释。APT-CEST 成像可能是 ICH 早期诊断的可靠非侵入性生物标志物。
