热带爪蟾微小RNA（包括变态期植物微小RNA样RNA）的鉴定与表达谱分析

Identification and expression-profiling of Xenopus tropicalis miRNAs including plant miRNA-like RNAs at metamorphosis.

作者信息

Hikosaka Akira, Takaya Kenji, Jinno Michiko, Kawahara Akira

机构信息

Division of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Kagamiyama 1-7-1, Higashi-Hiroshima 739-8521, Japan.

出版信息

FEBS Lett. 2007 Jun 26;581(16):3013-8. doi: 10.1016/j.febslet.2007.05.054. Epub 2007 May 29.

DOI:10.1016/j.febslet.2007.05.054

PMID:17544400

Abstract

Small RNAs (miR159-like RNAs) identical to some plant miR159s were found in Xenopus tropicalis miRNA cDNA libraries (30 miRNA families consisting of 75 unique sequences). Preferential expression of this RNA species was found in neural tissues during development. A sequence matching to this RNA species was not found in the 21 available animal's genome databases, but its resembling sequences associated with transposons were found in the X. tropicalis database. A possibility of horizontal transfer of the miR159 genes from plants will be discussed. Expression profiles of other miRNA species at metamorphosis were shown by DNA array and/or Northern hybridization.

摘要

在热带爪蟾的miRNA cDNA文库（由75个独特序列组成的30个miRNA家族）中发现了与某些植物miR159相同的小RNA（miR159样RNA）。在发育过程中，这种RNA种类在神经组织中优先表达。在21个可用的动物基因组数据库中未发现与这种RNA种类匹配的序列，但在热带爪蟾数据库中发现了与转座子相关的类似序列。将讨论miR159基因从植物水平转移的可能性。通过DNA阵列和/或Northern杂交展示了其他miRNA种类在变态过程中的表达谱。