苏丹染料色谱图中的“快速峰”
"Fast peaks" in chromatograms of Sudan dyes.
作者信息
Mölder Kadi, Künnapas Allan, Herodes Koit, Leito Ivo
机构信息
Institute of Chemical Physics, University of Tartu, Jakobi 2, 51014 Tartu, Estonia.
出版信息
J Chromatogr A. 2007 Aug 10;1160(1-2):227-34. doi: 10.1016/j.chroma.2007.05.052. Epub 2007 May 18.
The analysis of Sudan dyes has gained considerable attention during last years. In several publications multiple peaks in chromatograms of Sudan III and Sudan IV have been detected. It is demonstrated in this work that if the sample is kept in darkness before analysis, only one chromatographic peak appears for Sudan III or Sudan IV. In normal lighting conditions two peaks are observed. This light-induced effect can lead to under- or overestimation of the Sudan III and Sudan IV content by 10% and false-positive detection of Sudan I and Sudan II, if proper care is not taken. Appearance of the second peak in chromatograms of Sudan III and Sudan IV is attributed to photochromic E-Z isomerization of these dyes.
近年来，苏丹染料的分析受到了广泛关注。在多篇出版物中，已检测到苏丹III和苏丹IV色谱图中的多个峰。本研究表明，如果在分析前将样品避光保存，苏丹III或苏丹IV只会出现一个色谱峰。在正常光照条件下会观察到两个峰。如果不采取适当措施，这种光诱导效应可能导致苏丹III和苏丹IV含量被低估或高估10%，并导致苏丹I和苏丹II的假阳性检测。苏丹III和苏丹IV色谱图中第二个峰的出现归因于这些染料的光致变色E-Z异构化。
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